miR-98-5p 作为肝细胞癌预后生物标志物的临床应用研究。

Clinical application study on miR-98-5p as a prognostic biomarker in hepatocellular carcinoma.

机构信息

Department of Interventional Radiology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Huzhou, Zhejiang 313000, China.

Clinical Laboratory, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Huzhou, Zhejiang 313000, China.

出版信息

Clin Res Hepatol Gastroenterol. 2023 Feb;47(2):102077. doi: 10.1016/j.clinre.2023.102077. Epub 2023 Jan 6.

DOI:10.1016/j.clinre.2023.102077

PMID:36623770

Abstract

BACKGROUND

On the one hand, to investigate the targeted regulation of miR-98-5p on heparin-binding epidermal growth factor-like growth factor (HBEGF) in patients with hepatocellular carcinoma (HCC). On the other hand, elucidate the predictive effect of miR-98-5p combined with magnetic resonance imaging (MRI) data on the clinical prognosis of HCC patients.

METHODS

Serum samples from 98 HCC patients and 54 healthy subjects were selected in order to detect miR-98-5p as well as HBEGF expression levels via real-time quantitative PCR (RT-qPCR). A Luciferase reporter assay was performed to detect the interaction between miR-98-5p and HBEGF gene. The serum levels of IL-2, TNF-α, TGF-β1 and IFN-γ in HCC patients and in the control group (healthy subjects) were measured by enzyme-linked immunosorbent assay (ELISA). In addition, receiver operator characteristic curve (ROC) was utilized to analyze the predictive ability of miR-98-5p combined with HBEGF for HCC. Finally, the survival curves were used to analyze the effect of HBEGF and miR-98-5p on the survival of patients with HCC.

RESULTS

RT-qPCR results showed that the expression level of miR-98-5p was significantly decreased, while HBEGF expression was significantly increased in the serum of HCC patients compared with the control group. Luciferase reporter assay confirmed that miR-98-5p could target and bind HBEGF. Additionally, according to ELISA, IL-2, TNF-α, and TGF-β1 were significantly increased, while IFN-γ was significantly decreased in the serum of HCC patients compared with the control group. The results of ROC indicated that expressive levels of miR-98-5p and HBEGF had a high diagnostic value for HCC. At the same time, the survival curve results indicated high HBEGF expression and low miR-98-5p expression, suggesting a poor prognosis for HCC patients.

CONCLUSION

MiR-98-5p can target the down-regulating HBEGF gene. In addition, miR-98-5p combined with MRI data is of crucial guiding value in assessing the prognosis of patients with HCC in the clinic.

摘要

背景

一方面，研究 miR-98-5p 对肝癌（HCC）患者肝素结合表皮生长因子样生长因子（HBEGF）的靶向调节。另一方面，阐明 miR-98-5p 联合磁共振成像（MRI）数据对 HCC 患者临床预后的预测作用。

方法

选取 98 例 HCC 患者和 54 例健康对照者的血清样本，采用实时定量 PCR（RT-qPCR）检测 miR-98-5p 及 HBEGF 表达水平。采用荧光素酶报告基因实验检测 miR-98-5p 与 HBEGF 基因的相互作用。采用酶联免疫吸附试验（ELISA）检测 HCC 患者和对照组（健康对照者）血清中白细胞介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）和干扰素-γ（IFN-γ）的水平。此外，采用受试者工作特征曲线（ROC）分析 miR-98-5p 联合 HBEGF 对 HCC 的预测能力。最后，采用生存曲线分析 HBEGF 和 miR-98-5p 对 HCC 患者生存的影响。

结果

RT-qPCR 结果显示，与对照组相比，HCC 患者血清中 miR-98-5p 的表达水平明显降低，而 HBEGF 的表达水平明显升高。荧光素酶报告基因实验证实 miR-98-5p 可靶向结合 HBEGF。此外，根据 ELISA 结果，与对照组相比，HCC 患者血清中 IL-2、TNF-α 和 TGF-β1 水平明显升高，IFN-γ 水平明显降低。ROC 结果表明，miR-98-5p 和 HBEGF 的表达水平对 HCC 具有较高的诊断价值。同时，生存曲线结果表明，HBEGF 表达较高而 miR-98-5p 表达较低的患者 HCC 预后较差。