The effect of hydroalcoholic extract of leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke.

OBJECTIVE

Cigarette smoke (CS) contains compounds such as reactive oxygen species (ROS). Oxidative stress caused by excessive ROS eventually leads to germ cell apoptosis and male infertility. The leaves of (chicory) are rich in natural antioxidants, but their protective effects on the adverse effects of CS on testicular tissue have not been studied.

MATERIALS AND METHODS

24 Wistar rats were classified into four groups: control, extract: treatment with chicory extract (200 mg/kg body weight/day) for 13 weeks, smoke: exposed to CS for 13 weeks, and smoke + extract: exposed to CS and treated with the C. extract. Histological and biochemical analyses and apoptosis assay were done, and and expression was determined.

RESULTS

Treatment with compensated for the reduction of Sertoli cells, spermatogonia, spermatocytes, and spermatids caused by CS. Chicory extract reduced free radicals and improved antioxidant status. The lowest and highest percentage of apoptotic cells was observed in the extract and smoke groups, respectively, while simultaneous treatment with extract led to a significant reduction of apoptotic cells. The mean levels in the control, extract, smoke and smoke + extract groups were 1.00±0.57, 1.93±0.25, 5.98±0.42, and 0.62±0.22, respectively (p˂0.05). The mean levels of expression in the control, extract, smoke and smoke + extract groups were 1.00±0.31, 2.28±0.65, 5.55±0.40, and 0.21±0.23 (p˂0.05).

CONCLUSION

The extract probably affected expression by downregulation of These led to a decrease in free radicals and apoptosis, and an improvement in antioxidant status.