Converting pH probes into "turn-on" fluorescent receptors for anions.

Recognition of anions by synthetic receptors is an integral part of supramolecular chemistry continuing to expand and find new application areas in our daily life. Many applications require visualization of anion recognition events, and the generated analytical signal is used to quantify anions in solution. Transferring a binding event to a measured signal is a challenging task. The design of a synthetic receptor must involve not only the perfectly positioned binding sites with complementary noncovalent interactions for a guest but should also realize the sensing mechanism that generates a strong analytical response upon guest binding. This feature article outlines the design concept for the construction of "turn-on" fluorescent receptors for anions involving fluorescent pH probes. Applications of this concept for the construction of synthetic fluorescent receptors for inorganic anions and nucleotides are described. Features of the obtained receptors and possible competing binding and sensing processes in solution are analyzed to understand the scope and limitations of the approach.