长链非编码 RNA 和 microRNAs 簇在调控卵巢癌细胞中一系列肿瘤相关基因的表达水平中的作用。

Long Non-Coding RNAs and microRNAs Groups in the Regulation of Expression Level of a Number of Tumor-Associated Genes in Ovarian Cancer.

机构信息

Research Institute of General Pathology and Pathophysiology, Moscow, Russia.

N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2023 Jan;174(3):354-359. doi: 10.1007/s10517-023-05707-3. Epub 2023 Feb 1.

DOI:10.1007/s10517-023-05707-3

PMID:36723744

Abstract

The search for interacting long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs of protein-coding genes through the mechanism of competing endogenous RNAs in tumors of ovarian cancer patients was carried out. The levels of expression of 24 lncRNAs, 20 miRNAs, and 28 mRNAs of protein-coding genes involved in oncogenesis were determined by real-time PCR on a set of representative samples. Correlations between lncRNAs/miRNA and miRNA/mRNA levels in ovarian cancer samples were analyzed. We identified 8 pairs of lncRNAs/miRNA and 17 pairs of miRNA/mRNA, the expression levels of which have a negative correlation. Five triplets of potentially interacting lncRNAs/miRNA/mRNA have been identified, among which the most significant triplet is the OIP5-AS1/miR-203a-3p/ZEB1. The data obtained determine new epigenetic profiles, as well as new potential biomarkers and targets for targeted therapy of ovarian cancer patients.

摘要

通过竞争内源性 RNA 机制，在卵巢癌患者的肿瘤中寻找相互作用的长链非编码 RNA（lncRNA）、microRNA（miRNA）和蛋白质编码基因的 mRNAs。通过实时 PCR 在一组代表性样本上测定了 24 个 lncRNA、20 个 miRNA 和 28 个涉及致癌作用的蛋白质编码基因的 mRNA 的表达水平。分析了卵巢癌样本中 lncRNA/miRNA 和 miRNA/mRNA 水平之间的相关性。我们鉴定了 8 对 lncRNA/miRNA 和 17 对 miRNA/mRNA，其表达水平呈负相关。已经鉴定出 5 对潜在相互作用的 lncRNA/miRNA/mRNA ，其中最显著的是 OIP5-AS1/miR-203a-3p/ZEB1。获得的数据确定了新的表观遗传谱，以及新的潜在生物标志物和卵巢癌患者靶向治疗的靶点。