长链非编码 RNA MYU 通过海绵吸附 miR-6827-5p 并上调 HMGA1 促进卵巢癌细胞增殖。

Long non-coding RNA MYU promotes ovarian cancer cell proliferation by sponging miR-6827-5p and upregulating HMGA1.

机构信息

Department of Obstetrics and Gynecology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.

Department of Obstetrics and Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China.

出版信息

Pathol Oncol Res. 2023 Jan 27;29:1610870. doi: 10.3389/pore.2023.1610870. eCollection 2023.

DOI:10.3389/pore.2023.1610870

PMID:36776216

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9911462/

Abstract

Long non-coding RNAs (lncRNAs) have been confirmed to play vital roles in tumorigenesis. LncRNA MYU has recently been reported as an oncogene in several kinds of tumors. However, MYU's expression status and potential involvement in ovarian cancer (OC) remain unclear. In this study, we explored the underlying role of MYU in OC. The expression of MYU was upregulated in OC tissues, and MYU's overexpression was significantly correlated with the FIGO stage and lymphatic metastasis. Knockdown of MYU inhibited cell proliferation in SKOV3 and A2780 cells. Mechanistically, MYU directly interacted with miR-6827-5p in OC cells; HMGA1 is a downstream target gene of miR-6827-5p. Furthermore, MYU knockdown increased the expression of miR-6827-5p and decreased the expression of HMGA1. Restoration of HMGA1 expression reversed the influence on cell proliferation caused by MYU knockdown. MYU functions as a ceRNA that positively regulates HMGA1 expression by sponging miR-6827-5p in OC cells, which may provide a potential target and biomarker for the diagnosis or prognosis of OC.

摘要

长链非编码 RNA（lncRNA）已被证实在肿瘤发生中发挥重要作用。lncRNA MYU 最近在几种肿瘤中被报道为癌基因。然而，MYU 在卵巢癌（OC）中的表达状态及其潜在作用仍不清楚。在本研究中，我们探讨了 MYU 在 OC 中的潜在作用。MYU 在 OC 组织中的表达上调，并且 MYU 的过表达与 FIGO 分期和淋巴转移显著相关。在 SKOV3 和 A2780 细胞中，敲低 MYU 抑制细胞增殖。机制上，MYU 在 OC 细胞中直接与 miR-6827-5p 相互作用；HMGA1 是 miR-6827-5p 的下游靶基因。此外，MYU 敲低增加了 miR-6827-5p 的表达，降低了 HMGA1 的表达。HMGA1 表达的恢复逆转了 MYU 敲低对细胞增殖的影响。MYU 通过海绵吸附 miR-6827-5p 在 OC 细胞中正向调节 HMGA1 表达，这可能为 OC 的诊断或预后提供潜在的靶点和生物标志物。