血清 Rcn3 水平是结缔组织病相关间质性肺病的潜在诊断生物标志物，反映了肺功能的严重程度。

Serum Rcn3 level is a potential diagnostic biomarker for connective tissue disease-associated interstitial lung disease and reflects the severity of pulmonary function.

机构信息

Department of Respiratory and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, N0.5 Jingyuan Road, Beijing, 100043, China.

Department of Respiratory and Critical Care Medicine, Beijing Youan Hospital, Capital Medical University, No.8 Xi Tou Tiao, Youanmen Wai, Beijing, 100069, China.

出版信息

BMC Pulm Med. 2023 Feb 19;23(1):68. doi: 10.1186/s12890-023-02360-4.

DOI:10.1186/s12890-023-02360-4

PMID:36800954

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9938976/

Abstract

BACKGROUND

Although reticulocalbin 3 (Rcn3) has a critical role in alveolar epithelial function as well as in pathogenesis of pulmonary fibrosis, no study has yet examined its diagnostic and prognostic values for interstitial lung disease (ILD). This study aimed to evaluate Rcn3 as a potential marker in differential diagnosis of idiopathic pulmonary fibrosis (IPF) and connective tissue disease-associated interstitial lung disease (CTD-ILD) and in reflecting the severity of disease.

METHODS

This was a retrospective observational pilot study included 71 ILD patients and 39 healthy controls. These patients were stratified into IPF group (39) and CTD-ILD group (32). The severity of ILD was evaluated through pulmonary function test.

RESULTS

Serum Rcn3 level was statistically higher in CTD-ILD patients than that in IPF patients (p = 0.017) and healthy controls (p = 0.010). Serum Rcn3 further showed statistically negative correlation with pulmonary function indexes (TLC% pred and DLCO% pred) and positive correlation with inflammatory indexes (CRP and ESR) (r = - 0.367, p = 0.039; r = - 0.370, p = 0.037; r = 0.355, p = 0.046; r = 0.392, p = 0.026, respectively) in CTD-ILD patients rather than IPF patients. ROC analysis demonstrated that serum Rcn3 had superior diagnostic value for CTD-ILD and a cutoff value of 2.73 ng/mL had a sensitivity of 69%, a specificity of 69% and an accuracy of 45% for diagnose of CTD-ILD.

CONCLUSIONS

Serum Rcn3 levels might be a clinically useful biomarker in screening and evaluating CTD-ILD.

摘要

背景

尽管网状钙结合蛋白 3（Rcn3）在肺泡上皮功能以及肺纤维化发病机制中起着关键作用，但尚无研究检查其对间质性肺病（ILD）的诊断和预后价值。本研究旨在评估 Rcn3 作为特发性肺纤维化（IPF）和结缔组织疾病相关间质性肺病（CTD-ILD）鉴别诊断的潜在标志物，并反映疾病的严重程度。

方法

这是一项回顾性观察性试点研究，共纳入 71 例ILD 患者和 39 名健康对照者。这些患者分为 IPF 组（39 例）和 CTD-ILD 组（32 例）。ILD 的严重程度通过肺功能测试进行评估。

结果

CTD-ILD 患者的血清 Rcn3 水平明显高于 IPF 患者（p = 0.017）和健康对照组（p = 0.010）。血清 Rcn3 与肺功能指标（TLC%pred 和 DLCO%pred）呈统计学负相关，与炎症指标（CRP 和 ESR）呈统计学正相关（r = - 0.367，p = 0.039；r = - 0.370，p = 0.037；r = 0.355，p = 0.046；r = 0.392，p = 0.026），而在 IPF 患者中无此相关性。ROC 分析表明，血清 Rcn3 对 CTD-ILD 具有较好的诊断价值，其诊断 CTD-ILD 的截断值为 2.73ng/ml，灵敏度为 69%，特异性为 69%，准确性为 45%。