基于有丝分裂原刺激细胞因子产生和线粒体 DNA 指标对阿尔茨海默病患者进行免疫评估。

Immunological evaluation of patients with Alzheimer's disease based on mitogen-stimulated cytokine productions and mitochondrial DNA indicators.

机构信息

Department of Laboratory Medicine, College of Health Science and Technology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, One Health Center, Shanghai Jiao Tong University-The University of Edinburgh, Shanghai, China.

出版信息

BMC Psychiatry. 2023 Mar 8;23(1):145. doi: 10.1186/s12888-023-04634-x.

DOI:10.1186/s12888-023-04634-x

PMID:36890488

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9993804/

Abstract

BACKGROUND

Based on its objective characteristics, laboratory markers have always been the research direction of clinical diagnosis and assessment of mental disorders including Alzheimer's disease.

METHODS

MTT Colorimetric Assay, ELISA, and quantitative PCR were used to investigate the responsiveness of peripheral blood mononuclear cells (PBMCs) to mitogen Lipopolysaccharides (LPS) and Phytohemagglutinin (PHA), PBMCs genomic methylation and hydroxymethylation levels, nuclear DNA and mitochondrial DNA damage, respiratory chain enzyme activities, and circulating cell-free mitochondrial DNA levels were detected in 90 patients with Alzheimer's disease.

RESULTS

In the Alzheimer's disease group, LPS stimulated PBMCs viability, TNF-α secretion, PHA stimulated IL-10 secretion, genomic DNA methylation levels, circulating cell-free mitochondrial DNA copies, citrate synthase activity were reduced compared to the control; while the LPS stimulated PBMCs IL-1α secretion, PHA stimulated IL-1α and IFN-γ secretion, plasma IL-6 and TNF-α, mitochondrial DNA damages were increased compared to the control.

CONCLUSIONS

The reactivity of peripheral blood mononuclear cells to mitogens, mitochondrial DNA integrity characteristics, and cell-free mitochondrial DNA copies may be used as candidate laboratory biomarkers to help clinical management of Alzheimer's disease.

摘要

背景

基于其客观特征，实验室标志物一直是包括阿尔茨海默病在内的精神障碍临床诊断和评估的研究方向。

方法

采用 MTT 比色法、ELISA 和定量 PCR 检测 90 例阿尔茨海默病患者外周血单个核细胞（PBMC）对有丝分裂原脂多糖（LPS）和植物血凝素（PHA）的反应性、PBMC 基因组甲基化和羟甲基化水平、核 DNA 和线粒体 DNA 损伤、呼吸链酶活性以及循环细胞游离线粒体 DNA 水平。

结果

与对照组相比，阿尔茨海默病组 LPS 刺激 PBMC 活力、TNF-α 分泌、PHA 刺激 IL-10 分泌、基因组 DNA 甲基化水平、循环细胞游离线粒体 DNA 拷贝数、柠檬酸合酶活性降低；而 LPS 刺激 PBMC 中 IL-1α 分泌、PHA 刺激 IL-1α 和 IFN-γ 分泌、血浆中 IL-6 和 TNF-α、线粒体 DNA 损伤增加。