基于16S rRNA测序和靶向代谢组学研究分娩对荷斯坦奶牛瘤胃细菌和短链脂肪酸的影响
Influence of Parturition on Rumen Bacteria and SCFAs in Holstein Cows Based on 16S rRNA Sequencing and Targeted Metabolomics.
作者信息
Guo Yansheng, Wang Feifei, Mao Yongxia, Kong Weiyi, Wang Jiandong, Zhang Guijie
机构信息
College of Agriculture, Ningxia University, Yinchuan 750021, China.
Institute of Animal Sciences, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China.
出版信息
Animals (Basel). 2023 Feb 21;13(5):782. doi: 10.3390/ani13050782.
The rumen fluids from ten cows at Day 3~5 before calving and Day 0 after calving were collected to analyze the composition and quantity of bacterial communities and concentrations of SCFAs. The results showed that the relative abundances of unidentified and genera were significant increased ( < 0.05), while that of unidentified was notably decreased after calving ( < 0.05). In addition, the concentrations of acetic acid, propionic acid, butyric acid, and caproic acid obviously decreased after calving ( < 0.01). Our findings show that parturition altered the rumen microbiota and their fermentation ability in dairy cows. This study defines a rumen bacteria and metabolic profile of SCFAs associated with parturition in dairy cows.
采集了10头奶牛在产犊前3至5天和产犊后第0天的瘤胃液，以分析细菌群落的组成和数量以及短链脂肪酸的浓度。结果表明，未鉴定属和某属的相对丰度显著增加（P<0.05），而未鉴定属的相对丰度在产犊后显著降低（P<0.05）。此外，产犊后乙酸、丙酸、丁酸和己酸的浓度明显降低（P<0.01）。我们的研究结果表明，分娩改变了奶牛瘤胃微生物群及其发酵能力。本研究确定了与奶牛分娩相关的瘤胃细菌和短链脂肪酸代谢谱。
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