水-甲醇粗提物及（伞形科）叶片的溶剂提取物对小鼠的抗糖尿病活性
Anti-Diabetic Activities of Hydro-Methanolic Crude Extract and Solvent Fractions of (Apiaceae) Leaves in Mice.
作者信息
Zeleke Yeshiwas Guadie, Atnafie Seyfe Asrade, Aragaw Tezera Jemere
机构信息
College of Medicine and Health Sciences Comprehensive Specialized Hospital, University of Gondar, Gondar, Ethiopia.
Department of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
出版信息
J Exp Pharmacol. 2023 Mar 10;15:107-121. doi: 10.2147/JEP.S392742. eCollection 2023.
BACKGROUND
has been used to treat diabetes traditionally. There was no in vivo study to support the claim. This study aimed to confirm anti-diabetic activity of 80% methanol in water extract and solvent fractions of leaves in mice.
METHODS
leaves were macerated and extracted in 80% methanol in water. Hydro-methanol extract of leaves were tested in mice models. Overnight fasted mice were randomly divided into five groups for normoglycemic and glucose-loaded models as a negative control, positive control, and three tested groups, whereas, in streptozotocin-induced diabetic models, the mice were grouped into six groups each comprised six mice: diabetic negative control and normal negative control groups treated with 10 mL/kg distilled water, diabetic positive control group treated with Glibenclamide 5 mg/kg and three diabetic tested groups treated with extract at 100, 200, and 400 mg/kg doses. A one-way ANOVA was performed to analyze the data, and the post hoc Tukey's test was utilized for multiple comparisons. The P-value <0.05 was considered statistically significant.
RESULTS
Hydro-methanol extract of leaves at 400 mg/kg in normoglycemic mice significantly lowered blood glucose levels (BGLs) (P< 0.01). Mice with oral glucose-loaded test lowered BGLs at dosages of 200 mg/kg (P < 0.05) and 400 mg/kg (P < 0.01) respectively. Single-dose of ethyl acetate, n-hexane fractions and hydro-methanol extract at 100 mg/kg, 400 mg/kg and 200 mg/kg reduced BGLs (P < 0.05, P < 0.001, and P < 0.01) respectively. BGL drops in diabetic mice given daily repeated doses of 200 mg/kg of hydro-methanol extract and 400 mg/kg of ethyl acetate fraction (P < 0.001). Diabetic mice gained weight at a 400 mg/kg hydro-methanol extract and ethyl acetate fraction (P < 0.05 and P < 0.01) respectively. Hydro-methanol extract and ethyl acetate fraction and at 200 mg/kg decreased total cholesterol, triglycerides, and low-density lipoprotein and increased high-density lipoprotein (P < 0.001).
CONCLUSION
80% methanol in water extract and solvent fractions of leaves showed anti-diabetic effects and significantly reduced hyperlipidemia in diabetics, this study supported the traditional usage of for treating diabetes; however, species variation could also limit such a straightforward extrapolation of the findings of this study in humans.
背景
传统上一直被用于治疗糖尿病。但尚无体内研究支持这一说法。本研究旨在证实叶的80%甲醇水提取物及其溶剂部位在小鼠体内的抗糖尿病活性。
方法
将叶进行浸渍并用80%甲醇水提取。叶的氢甲醇提取物在小鼠模型中进行测试。过夜禁食的小鼠被随机分为五组，用于正常血糖和葡萄糖负荷模型，分别作为阴性对照、阳性对照和三个测试组，而在链脲佐菌素诱导的糖尿病模型中，小鼠被分为六组，每组六只：糖尿病阴性对照组和正常阴性对照组用10 mL/kg蒸馏水治疗，糖尿病阳性对照组用格列本脲5 mg/kg治疗，三个糖尿病测试组分别用100、200和400 mg/kg剂量的提取物治疗。采用单因素方差分析来分析数据，并使用事后Tukey检验进行多重比较。P值<0.05被认为具有统计学意义。
结果
在正常血糖小鼠中，400 mg/kg叶的氢甲醇提取物显著降低血糖水平（BGLs）（P<0.01）。口服葡萄糖负荷试验的小鼠分别在200 mg/kg（P<0.05）和400 mg/kg（P<0.01）剂量下降低了BGLs。单剂量的乙酸乙酯、正己烷部位以及100 mg/kg、400 mg/kg和200 mg/kg的氢甲醇提取物分别降低了BGLs（P<0.05、P<0.001和P<0.01）。每天重复给予200 mg/kg氢甲醇提取物和400 mg/kg乙酸乙酯部位的糖尿病小鼠血糖下降（P<0.001）。糖尿病小鼠分别在400 mg/kg氢甲醇提取物和乙酸乙酯部位体重增加（P<0.05和P<0.01）。200 mg/kg的氢甲醇提取物和乙酸乙酯部位降低了总胆固醇、甘油三酯和低密度脂蛋白，并增加了高密度脂蛋白（P<0.001）。
结论
叶的80%甲醇水提取物及其溶剂部位显示出抗糖尿病作用，并显著降低糖尿病患者的高脂血症，本研究支持了传统上用于治疗糖尿病的用途；然而，物种差异也可能限制将本研究结果直接外推至人类。
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