Micro- and nanoplastic toxicity: A review on size, type, source, and test-organism implications.

Polymeric wastes are among the current major environmental problems due to potential pollution and contamination. Within the spectrum of polymeric waste, microplastics (MPs) and nanoplastics (NPs) have gained ground in recent research since these particles can affect the local biota, inducing toxic effects on several organisms. Different outcomes have been reported depending on particle sizes, shape, types, and exposed organisms and conditions, among other variables. This review aimed to compile and discuss the current knowledge and possible literature gaps regarding the MPs and NPs generation and their toxicological effects as stressors, considering polymer type (as polyethylene, polypropylene, polyethylene terephthalate, polystyrene, polyvinyl chloride, or others), size (micro- or nano-scale), source (commercial, lab-synthesized, or environmental) and test organism group. In that sense, 615 publications were analyzed, among which 72 % discussed micro-sized plastics, while <28 % assayed the toxicity of NPs (<1 μm). For most polymers, MPs and NPs were commercially purchased and used without additional size reduction processes; except for polyethylene terephthalate studies that mostly used grinding and cutting methods to obtain MPs. Polystyrene (PS) was the main polymer studied, as both MPs and NPs. PS accounts for >90 % of NPs reports evaluated, reflecting a major literature gap if compared to its 35.3 % share on MPs studies. Among the main organisms, arthropods and fish combined accounted for nearly 40 % of toxicity testing. Overall, the different types of plastics showed a tendency to report toxic effects, except for the 'Survival/lethality' category, which might indicate that polymeric particles induce mostly sublethal toxic effects. Furthermore, despite differences in publication numbers, we observed greater toxicity reported for NPs than MPs with oxidative stress among the majorly investigated endpoints. This study allowed a hazard profile overview of micro/nanoplastics (MNPs) and the visualization of literature gaps, under a broad diversity of toxicological evidence.