绿茶提取物可增强视神经损伤大鼠的视网膜神经节细胞存活和轴突再生。

Green tea extract enhances retinal ganglion cell survival and axonal regeneration in rats with optic nerve injury.

机构信息

Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China; Shantou University Medical College, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.

出版信息

J Nutr Biochem. 2023 Jul;117:109333. doi: 10.1016/j.jnutbio.2023.109333. Epub 2023 Mar 24.

DOI:10.1016/j.jnutbio.2023.109333

PMID:36965783

Abstract

Current clinical treatments have not yet effectively cured progressive retinal ganglion cell (RGC) death and axonal degeneration after optic nerve (ON) injury. We previously demonstrated green tea extract (GTE) can reduce RGC death in rats after ischemic injury. Here, we aim to determine the prophylactic and therapeutic effects and mechanisms of GTE on RGC survival and axonal regeneration in rats with ON injury. GTE (275 or 550 mg/kg) was administered intragastrically for 7 d before or 14 d post-ON crush surgery in adult Fischer 344 rats. Rats with pre- or post-operative treatment of 275 mg/kg GTE showed significantly higher numbers of RGCs and regenerated axons post-ON injury with improved pupillary light reflex as compared to saline-treated rats. Akt and Erk p42/44 activation was higher in the retina of rats given 275 mg/kg GTE pre-surgery, whereas Stat3 activation was higher in those with 275 mg/kg GTE post-operation. Less activated microglia were observed in rats with pre-treatment of 275 or 550 mg/kg GTE. RNA sequencing analysis identified the downregulation of inflammation, apoptosis, and microglia activation genes in the retina of rats with pre- or post-treatment with 275 mg/kg GTE as compared to the saline-treated rats. In summary, this study revealed the prophylactic and therapeutic treatment effects of GTE on RGC survival and axonal regeneration in rats with ON injury, indicating a potential alternative treatment for traumatic optic neuropathy.

摘要

目前的临床治疗方法尚未有效治愈视神经（ON）损伤后进行性视网膜神经节细胞（RGC）死亡和轴突变性。我们之前的研究表明，绿茶提取物（GTE）可以减少大鼠缺血性损伤后 RGC 的死亡。在此，我们旨在确定 GTE 对 ON 损伤大鼠 RGC 存活和轴突再生的预防和治疗作用及其机制。在成年 Fischer 344 大鼠 ON 挤压手术后 7 天或 14 天前，通过胃内给予 GTE（275 或 550mg/kg）。与生理盐水处理的大鼠相比，术前给予 275mg/kg GTE 或术后给予 GTE 的大鼠，ON 损伤后 RGC 数量和再生轴突明显增多，瞳孔光反射改善。与生理盐水处理的大鼠相比，术前给予 275mg/kg GTE 的大鼠视网膜中 Akt 和 Erk p42/44 的激活更高，而术后给予 275mg/kg GTE 的大鼠中 Stat3 的激活更高。在术前给予 275 或 550mg/kg GTE 的大鼠中，观察到激活的小胶质细胞减少。与生理盐水处理的大鼠相比，术前或术后给予 275mg/kg GTE 的大鼠视网膜中炎症、细胞凋亡和小胶质细胞激活基因的下调。综上所述，本研究揭示了 GTE 对 ON 损伤大鼠 RGC 存活和轴突再生的预防和治疗作用，表明其可能是外伤性视神经病变的一种潜在替代治疗方法。