Antibiotic use in infants within the first year of life is associated with the appearance of antibiotic-resistant genes in their feces.

BACKGROUND

Antibiotic resistance, an increasing challenge, is not only a national threat but also a global threat. Carriage of resistance genes is not limited to adults alone, various microbiota niches present in the body system of children have been found to harbor bacteria carrying resistant genes, especially, their gut microbiota. This study aims to identify selected antibiotic-resistant genes from the fecal samples of infants and the association of antibiotics use with the occurrence of resistant genes in the infant's gut.

METHODS

A total number of 172 metagenomic DNA samples previously extracted from stool samples of 28 Nigerian babies longitudinally within their first year of life were screened for the presence of ESBL genes (SHV, TEM, and CTX-M), PMQR genes (, , , ), ribosomal protection protein tetracycline resistance gene, (RPP) β-lactamase (), macrolide (, , A/E), aminoglycoside modifying enzymes gent (aac(6')/aph(2″)) and genes by PCR. Nineteen (19) of the 28 babies used antibiotics during the study. The association between antibiotic use by the babies within the first year of life and occurrence of resistant genes were analyzed by Spearman rank correlation.

RESULTS

One hundred and twenty-two (122) samples (71%) out of the 172 isolates had antibiotic-resistance genes. PMQR genes were absent in all the samples. Three isolates had TEM gene, nine isolates had SHV gene, six isolates had CTX-M gene and 19 isolates had gene, 31 samples had gene, 29 samples had gene, 27 samples had gene, four samples had gene, 13 samples had gene and 16 samples had gene. The babies whose samples had resistant genes used antibiotics in the same months the samples were collected. Interestingly, the 11 babies whose samples had the A gene all used antibiotics in the same months their samples were collected but none of them used trimethoprim/sulfamethoxazole antibiotic. The overall correlation matrix of the babies showed a strong association between antibiotic use (AU) and antibiotic use presence of resistance genes (AUPRG) with a coefficient of 0.89. Antibiotic-resistant genes are present in the gut of infants and their occurrence is strongly connected with antibiotic use by infants.