戊型肝炎病毒细胞培养。

HEV Cell Culture.

机构信息

Division of Therapeutical Monoclonal Antibodies, National Institutes for Food and Drug Control, Beijing, China.

Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Kunming, Yunnan Province, China.

出版信息

Adv Exp Med Biol. 2023;1417:119-131. doi: 10.1007/978-981-99-1304-6_8.

DOI:10.1007/978-981-99-1304-6_8

PMID:37223862

Abstract

Cell culture is an important research method in virology. Although many attempts have been conducted to culture HEV in cells, only a few cell culture systems were considered to be efficient enough for usage. Concentration of virus stocks, host cells, and medium components affects the culture efficiency and the genetic mutations during HEV passage were found to be associated with the increased virulence in cell culture. As an alternative method for traditional cell culture, the infectious cDNA clones were constructed. The viral thermal stability, factors that impact the host range, post-translation of viral proteins, and function of different viral proteins were studied using the infectious cDNA clones. HEV cell culture studies on progeny virus showed that the viruses secreted from host cells have an envelope and its formation was associated with pORF3. This result explained the phenomenon that virus could infect host cells in the presence of anti-HEV antibodies.

摘要

细胞培养是病毒学中的一种重要研究方法。尽管已经进行了许多尝试来在细胞中培养 HEV，但只有少数细胞培养系统被认为足够高效以供使用。病毒滴度、宿主细胞和培养基成分的浓度会影响培养效率，并且在 HEV 传代过程中发现的遗传突变与细胞培养中的毒力增加有关。作为传统细胞培养的替代方法，构建了传染性 cDNA 克隆。使用传染性 cDNA 克隆研究了病毒的热稳定性、影响宿主范围的因素、病毒蛋白的翻译后修饰以及不同病毒蛋白的功能。对后代病毒的 HEV 细胞培养研究表明，从宿主细胞中分泌的病毒具有包膜，其形成与 pORF3 有关。这一结果解释了在存在抗 HEV 抗体的情况下病毒仍能感染宿主细胞的现象。