评估褪黑素对暴露于乙酰胺腙的雌性小鼠卵母细胞、胚胎和卵巢组织参数的保护作用。

Evaluation of the protective effect of melatonin on oocyte, embryo and ovarian tissue parameters in female mice exposed to acetamiprid.

机构信息

Student of Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Professor of Anatomical Sciences Department of Anatomical Sciences, School of Medicine, Infertility and Reproductive Health Research Center Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

出版信息

JBRA Assist Reprod. 2023 Sep 12;27(3):407-413. doi: 10.5935/1518-0557.20220068.

DOI:10.5935/1518-0557.20220068

PMID:37257062

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10712808/

Abstract

OBJECTIVE

Acetamiprid (ACP) causes infertility and its effect appears to occur via oxidative stress. Melatonin has antioxidative properties. Thus, in this experimental study, we examined the protective effect of melatonin against toxic pathologic changes from ACP on reproductive system parameters of female mice.

METHODS

The study included 30 female mice divided into 5 groups (6 mice in each group), as follows: Saline (control group); ACP (10, 20 mg/kg); ACP (10mg/kg) + melatonin (10mg/kg); and ACP (20mg/kg) + melatonin (10mg/kg). All mice were given intraperitoneal injections daily for one month. The groups were evaluated for ovarian histopathological changes and oocyte quality based on in vitro fertilization (IVF) parameters.

RESULTS

ACP induced histological damages in the ovaries of mice and caused increases in the number of atretic follicles and decreases in the quality of oocytes based on IVF parameters. These alterations were significantly reduced by melatonin.

CONCLUSIONS

Melatonin can decrease the toxic effects of ACP in the female reproductive system of mice. Further studies are needed.

摘要

目的

乙酰甲胺磷（ACP）可导致不孕，其作用似乎是通过氧化应激发生的。褪黑素具有抗氧化特性。因此，在这项实验研究中，我们研究了褪黑素对雌性小鼠生殖系统参数的 ACP 毒性病理变化的保护作用。

方法

该研究包括 30 只雌性小鼠，分为 5 组（每组 6 只），如下：生理盐水（对照组）；乙酰甲胺磷（10、20mg/kg）；乙酰甲胺磷（10mg/kg）+褪黑素（10mg/kg）；和乙酰甲胺磷（20mg/kg）+褪黑素（10mg/kg）。所有小鼠均每日腹腔注射，持续一个月。根据体外受精（IVF）参数评估各组的卵巢组织病理学变化和卵母细胞质量。