超越 DNA：用于 PAINT 超分辨率显微镜的新探针。
Beyond DNA: new probes for PAINT super-resolution microscopy.
机构信息
Department of Biomedical Engineering, Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands.
Department of Chemical Engineering and Chemistry, Laboratory of Self-Organizing Soft Matter, Eindhoven University of Technology, Eindhoven, 5612 AP, The Netherlands.
出版信息
Chem Commun (Camb). 2023 Jul 4;59(54):8332-8342. doi: 10.1039/d3cc00757j.
In the last decade, point accumulation for imaging in nanoscale topography (PAINT) has emerged as a versatile tool for single-molecule localization microscopy (SMLM). Currently, DNA-PAINT is the most widely used, in which a transient stochastically binding DNA docking-imaging pair is used to reconstruct specific characteristics of biological or synthetic materials on a single-molecule level. Slowly, the need for PAINT probes that are not dependent on DNA has emerged. These probes can be based on (i) endogenous interactions, (ii) engineered binders, (iii) fusion proteins, or (iv) synthetic molecules and provide complementary applications for SMLM. Therefore, researchers have been expanding the PAINT toolbox with new probes. In this review, we provide an overview of the currently existing probes that go beyond DNA and their applications and challenges.
在过去的十年中，点积累成像技术（PAINT）已成为单分子定位显微镜（SMLM）的一种多功能工具。目前，DNA-PAINT 是应用最广泛的方法，其中使用瞬时随机结合的 DNA 对接成像对来重建生物或合成材料的特定特征在单分子水平上。慢慢地，人们对不依赖 DNA 的 PAINT 探针的需求也出现了。这些探针可以基于 (i) 内源性相互作用，(ii) 工程结合物，(iii) 融合蛋白，或 (iv) 合成分子，并为 SMLM 提供互补的应用。因此，研究人员一直在用新的探针扩展 PAINT 工具箱。在这篇综述中，我们概述了目前超越 DNA 的探针及其应用和挑战。
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