基于 ceRNA 的关联网络分析揭示了大豆油脂代谢。
Analysis of -Associated ceRNA Network Reveals Oil Metabolism in Soybean.
机构信息
College of Life Sciences, Jilin Agricultural University, 2888, Xincheng Street, Changchun City, Jilin Province 130117, China.
College of Agronomy, Jilin Agricultural University, 2888, Xincheng Street, Changchun City, Jilin Province 130117, China.
出版信息
J Agric Food Chem. 2023 Jun 28;71(25):9815-9825. doi: 10.1021/acs.jafc.3c00993. Epub 2023 Jun 13.
Soybean [ (Linn.) Merr.] is an important oil crop. Long noncoding RNAs (lncRNAs) play a variety of functions in plants. However, their function in the soybean oil synthesis pathway is yet to be uncovered. Here, the gene related to soybean oil synthesis was screened, and the full-length cDNA sequence of the lncRNA was obtained using rapid amplification of cDNA ends. Overexpression of increased the content of crude protein in seeds, decreased the content of oleic acid, and affected the content of alanine and arginine in free amino acids. RNA interference of the gene decreased the crude protein content in seeds. Quantitative real-time polymerase chain reaction analysis revealed that influenced the expression of associated with phosphatidylinositol metabolism by acting as a decoy for , thereby affecting the content of soybean oil. Our results provide insights into how lncRNA-mediated competing endogenous RNA regulatory networks are involved in soybean oil synthesis.
大豆（Glycine max）是一种重要的油料作物。长链非编码 RNA（lncRNA）在植物中发挥多种功能。然而，它们在大豆油脂合成途径中的功能尚未被揭示。在这里，筛选了与大豆油脂合成相关的基因，并通过快速扩增 cDNA 末端获得了 lncRNA 的全长 cDNA 序列。过表达 增加了种子中粗蛋白的含量，降低了油酸的含量，并影响了游离氨基酸中丙氨酸和精氨酸的含量。 基因的 RNA 干扰降低了种子中的粗蛋白含量。定量实时聚合酶链反应分析表明， 通过充当 的诱饵，影响与磷脂酰肌醇代谢相关的 的表达，从而影响大豆油的含量。我们的研究结果为 lncRNA 介导的竞争内源性 RNA 调控网络如何参与大豆油脂合成提供了新的见解。
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