原儿茶酸对链脲佐菌素诱导的糖尿病大鼠糖尿病视网膜病变的有益作用。
Beneficial effects of protocatechuic acid on diabetic retinopathy in streptozocin-induced diabetic rats.
作者信息
Wan Ling, Li Jun, Chen Wei-Bai, Wu Guo-Qiang
机构信息
Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, China.
Department of Ophthalmology, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu 610072, Sichuan Province, China.
出版信息
Int J Ophthalmol. 2023 Jun 18;16(6):855-862. doi: 10.18240/ijo.2023.06.04. eCollection 2023.
AIM
To determine the effects of protocatechuic acid (PCA) on streptozocin-induced diabetic retinopathy (DR) in rats.
METHODS
Wistar rats were given a 50 mg/kg intraperitoneal injection of streptozocin to induce diabetes. Animals were assigned randomly one of four groups (8 rats per group): control, diabetic, diabetic plus PCA (25 mg/kg·d), and diabetic plus PCA (50 mg/kg·d). After inducing diabetes, treatments were started one week later and continued for eight weeks. After the experiment, the rats were sacrificed, and their retinas were taken for biochemical and molecular analysis.
RESULTS
PCA administration diminished the blood glucose and glycated haemoglobin levels relative to the diabetic group. In diabetic rats, PCA lowered elevated levels of advanced glycosylated end products (AGEs) and receptor for AGEs (RAGE). In the retina of diabetic rats, PCA effectively decreased inflammatory cytokine, nuclear factor-κB, tumour necrosis factor-α, interleukin-1β, and vascular endothelial growth factor, and increased antioxidant markers glutathione, superoxide dismutase, and catalase.
CONCLUSION
The protective benefits of PCA against DR may be attributable to its suppression of the AGEs and RAGE and its antioxidant and anti-inflammatory properties.
目的
确定原儿茶酸（PCA）对链脲佐菌素诱导的大鼠糖尿病视网膜病变（DR）的影响。
方法
给Wistar大鼠腹腔注射50 mg/kg链脲佐菌素以诱导糖尿病。将动物随机分为四组（每组8只大鼠）：对照组、糖尿病组、糖尿病加PCA组（25 mg/kg·d）和糖尿病加PCA组（50 mg/kg·d）。诱导糖尿病后，一周后开始治疗并持续八周。实验结束后，处死大鼠，取其视网膜进行生化和分子分析。
结果
与糖尿病组相比,给予PCA可降低血糖和糖化血红蛋白水平。在糖尿病大鼠中，PCA降低了晚期糖基化终产物（AGEs）和AGE受体（RAGE）的升高水平。在糖尿病大鼠的视网膜中，PCA有效降低了炎性细胞因子、核因子-κB、肿瘤坏死因子-α、白细胞介素-1β和血管内皮生长因子，并增加了抗氧化标志物谷胱甘肽、超氧化物歧化酶和过氧化氢酶。
结论
PCA对DR的保护作用可能归因于其对AGEs和RAGE的抑制及其抗氧化和抗炎特性。
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