The Transcription Factor MbWRKY46 in (L.) Borkh Mediate Cold and Drought Stress Responses.

The living environment of plants is not static; as such, they will inevitably be threatened by various external factors for their growth and development. In order to ensure the healthy growth of plants, in addition to artificial interference, the most important and effective method is to rely on the role of transcription factors in the regulatory network of plant responses to abiotic stress. This study conducted bioinformatics analysis on the gene, which was obtained through gene cloning technology from (L.) Borkh, and found that the gene had a total length of 1068 bp and encodes 355 amino acids. The theoretical molecular weight (MW) of the MbWRKY46 protein was 39.76 kDa, the theoretical isoelectric point (pI) was 5.55, and the average hydrophilicity coefficient was -0.824. The subcellular localization results showed that it was located in the nucleus. After conducting stress resistance studies on it, it was found that the expression of was tissue specific, with the highest expression level in roots and old leaves. Low temperature and drought had a stronger induction effect on the expression of this gene. Under low temperature and drought treatment, the expression levels of several downstream genes related to low temperature and drought stress (, , , , , ) increased more significantly in transgenic . This indicated that gene can be induced to upregulate expression in under cold and water deficient environments. The results of this study have a certain reference value for the application of in low-temperature and drought response, and provide a theoretical basis for further research on its function in the future.