中国广东养鸭场耐甲氧西林金黄色葡萄球菌中 oprtA 阳性的发生和污染。
The occurrence and contamination of optrA-positive methicillin-resistant Staphylococcus aureus from duck farms in Guangdong, China.
机构信息
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, China.
出版信息
J Glob Antimicrob Resist. 2023 Dec;35:86-92. doi: 10.1016/j.jgar.2023.08.015. Epub 2023 Sep 9.
OBJECTIVES
Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), is an important zoonotic microorganism that increasingly causes public health concern worldwide. The objective of this study was to determine the prevalence and transmission of S. aureus in duck farms and evaluate its antimicrobial resistance and genetic characteristics.
METHODS
The samples associated with ducks, feeders, and the environment were collected on 14 duck farms from four areas in Guangdong, China, from 2020 to 2021. All isolates were subjected to antimicrobial susceptibility testing. A comprehensive epidemiological survey of S. aureus was conducted by S. aureus protein A typing and whole-genome sequencing.
RESULTS
A total of 560 samples were collected. The prevalence rate of MRSA among ducks (8.1%, 11 of 135) was higher compared with that in environmental samples. OptrA-positive ST398-t034 MRSA were first detected from duck farms in China. A total of 79.3% (34 of 46) S. aureus isolates showed multidrug-resistant phenotypes. Notably, some isolates carried multidrug-resistant genes encoding macrolide-lincosamide-streptogramin B, pleuromutilin-pleuromutilin-streptogramin A, and oxazolidinone. Analysis of the virulence genes revealed that the MRSA isolates carried genes encoding gamma-hemolysin, enterotoxin, and leukocidin. ST9-t899 is a primary clonal lineage among duck- and environment-associated MRSA. Single-nucleotide polymorphism analysis showed the potential contamination relationship of optrA-positive ST2308 MRSA isolates carrying the gamma-hemolysin genes and the leukocidin virulence genes between airborne dust and sick ducks.
CONCLUSION
The contamination of MRSA, especially optrA-positive MRSA, between food animals and the environment is a growing public health concern worldwide. Based on One Health principles, continuous surveillance of MRSA is urgently needed.
目的
金黄色葡萄球菌（尤其是耐甲氧西林金黄色葡萄球菌，MRSA）是一种重要的人畜共患病原体，在全球范围内日益引起公共卫生关注。本研究旨在确定鸭场金黄色葡萄球菌的流行情况和传播情况，并评估其抗菌药物耐药性和遗传特征。
方法
2020 年至 2021 年，从中国广东四个地区的 14 个鸭场采集与鸭子、饲料和环境相关的样本。对所有分离株进行抗菌药物敏感性试验。通过金黄色葡萄球菌蛋白 A 分型和全基因组测序对金黄色葡萄球菌进行全面的流行病学调查。
结果
共采集 560 份样本。鸭子中 MRSA 的检出率（8.1%，135 份中的 11 份）高于环境样本。首次从中国鸭场检测到 oprtA 阳性 ST398-t034 MRSA。46 株金黄色葡萄球菌分离株中 79.3%（34 株）表现出多药耐药表型。值得注意的是，一些分离株携带了编码大环内酯类-林可酰胺类-链阳性菌素 B、截短侧耳素-截短侧耳素-链阳性菌素 A 和恶唑烷酮类药物耐药基因。毒力基因分析显示，MRSA 分离株携带编码γ-溶血性毒素、肠毒素和白细胞毒素的基因。ST9-t899 是鸭源和环境源 MRSA 的主要克隆谱系。单核苷酸多态性分析表明，携带γ-溶血性毒素基因和白细胞毒素毒力基因的 oprtA 阳性 ST2308 MRSA 分离株之间存在空气传播尘埃和患病鸭子之间的潜在污染关系。
结论
食源性动物与环境之间耐甲氧西林金黄色葡萄球菌（尤其是 oprtA 阳性 MRSA）的污染是全球范围内日益严重的公共卫生问题。基于同一健康原则，迫切需要对耐甲氧西林金黄色葡萄球菌进行持续监测。
Zotero 插件