从. 的 57B 区域产生突变体。

Generation of Mutants from the 57B Region of .

机构信息

Developmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, Germany.

Zoology & Physiology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building B2.1, D-66123 Saarbrücken, Germany.

出版信息

Genes (Basel). 2023 Nov 6;14(11):2047. doi: 10.3390/genes14112047.

DOI:10.3390/genes14112047

PMID:38002990

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10671637/

Abstract

The 57B region of includes a cluster of the three homeobox genes (), (), and (). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for , , and . With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of , which could now be used to further explore the functions of the corresponding genes in the future.

摘要

包括三个同源盒基因（）、（）和（）在内的 57B 区域。为了分离这些基因的突变体，我们进行了 EMS 诱变，并从 57B 区域分离出致死突变体，其中包括、和的突变体。借助于来自 57B 区域的两个新生成的缺失，我们将额外的突变体定位到特定的染色体区间，并从 57B 区域分子上鉴定了其中的几个突变体。此外，我们通过基因靶向生成了 CG15651 和 RIC-3 的突变体，并且使用 CRISPR/Cas9 系统生成了 CG9344、CG15649、CG15650 和 ND-B14.7 基因的突变体。我们确定了大多数分离突变体在发育过程中的致死期。总共，我们分析了 57B 区域的九个不同基因的等位基因，这些基因的等位基因在未来可以进一步用于探索相应基因的功能。