利用污水和奶酪乳清生产聚羟基脂肪酸酯。

Production of polyhydroxyalkanoates using sewage and cheese whey.

作者信息

Chang Young-Cheol, Reddy M Venkateswar, Tsukiori Yusei, Mawatari Yasuteru, Choi DuBok

机构信息

Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, Hokkaido, 050-8585, Japan.

Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, CO, 80523, USA.

出版信息

Heliyon. 2023 Dec 2;9(12):e23130. doi: 10.1016/j.heliyon.2023.e23130. eCollection 2023 Dec.

DOI:10.1016/j.heliyon.2023.e23130

PMID:38144304

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10746463/

Abstract

Recently, polyhydroxyalkanoates (PHAs) have been produced using raw sewage in our laboratory; however, the production concentrations are low. Therefore, this study aimed to enhance PHA production by applying different strategies. PHA production was higher in sewage-containing medium than in mineral salt medium and was enhanced 22-fold after glucose supplementation. A relatively high degree of glucose consumption (83.6 ± 1.59 %) was also achieved. Bacteria incubated with cheese whey diluted with sewage showed higher PHA production than bacteria incubated with cheese whey diluted with distilled water did. The expression of the PHA synthase gene () was evaluated via real-time polymerase chain reaction using low- and high-carbon-containing sewage. Relatively higher expression levels were observed in high-carbon-containing sewage but at lower nitrogen concentrations. The characteristics of the produced PHA were comparable to those of standard PHA. Therefore, this study revealed that the bacterium sp. CYR1 can produce PHA from low- or high-carbon-containing wastewater.

摘要

最近，我们实验室已利用未经处理的污水生产聚羟基脂肪酸酯（PHA）；然而，生产浓度较低。因此，本研究旨在通过应用不同策略提高PHA产量。含污水培养基中的PHA产量高于矿物盐培养基，添加葡萄糖后产量提高了22倍。还实现了相对较高的葡萄糖消耗程度（83.6 ± 1.59%）。用污水稀释的奶酪乳清培养的细菌比用蒸馏水稀释的奶酪乳清培养的细菌表现出更高的PHA产量。通过使用低碳和高碳污水的实时聚合酶链反应评估PHA合酶基因（）的表达。在高碳污水中但在较低氮浓度下观察到相对较高的表达水平。所生产PHA的特性与标准PHA相当。因此，本研究表明，CYR1菌可从低碳或高碳废水中生产PHA。