一种用于检测铝/锌/镉的新型多功能荧光探针及其实际应用。
A Novel Multi-Functional Fluorescence Probe for the Detection of Al/Zn/Cd and its Practical Applications.
作者信息
Fan Jing, Liu Xiao-Meng, Sun Peng, Su Huan, Sun Yan, Li Qing-Zhong, Xie Cheng-Zhi, Xu Jing-Yuan
机构信息
Department of Chemical Biology and Tianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, P. R. China.
School of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, P. R. China.
出版信息
J Fluoresc. 2025 Feb;35(2):1025-1036. doi: 10.1007/s10895-024-03589-w. Epub 2024 Jan 22.
A novel multi-functional fluorescence probe HMIC based on hydrazide Schiff base has been successfully synthesized and characterized. It can distinguish Al/Zn/Cd in ethanol, in which fluorescence emission with different colors (blue for Al, orange for Zn, and green for Cd) were presented. The limits of detection of HMIC towards three ions were calculated from the titration curve as 7.70 × 10 M, 4.64 × 10 M, and 1.35 × 10 M, respectively. The structures of HMIC and its complexes were investigated using UV-Vis spectra, Job's plot, infrared spectra, mass spectrometry, H-NMR and DFT calculations. Practical application studies have also demonstrated that HMIC can be applied to real samples with a low impact of potential interferents. Cytotoxicity and cellular imaging assays have shown that HMIC has good cellular permeability and potential antitumor effects. Interestingly, HMIC can image Al, Zn and Cd in the cells with different fluorescence signals.
一种基于酰肼席夫碱的新型多功能荧光探针HMIC已成功合成并表征。它能在乙醇中区分铝离子（Al）、锌离子（Zn）和镉离子（Cd），呈现出不同颜色的荧光发射（铝离子为蓝色、锌离子为橙色、镉离子为绿色）。通过滴定曲线计算出HMIC对这三种离子的检测限分别为7.70×10⁻⁸ M、4.64×10⁻⁸ M和1.35×10⁻⁸ M。利用紫外可见光谱、乔布氏图、红外光谱、质谱、氢核磁共振谱和密度泛函理论计算研究了HMIC及其配合物的结构。实际应用研究还表明，HMIC可应用于实际样品，潜在干扰物的影响较小。细胞毒性和细胞成像分析表明，HMIC具有良好的细胞通透性和潜在的抗肿瘤作用。有趣的是，HMIC能在细胞中以不同的荧光信号对铝、锌和镉进行成像。
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