韦氏梭菌分泌的阿魏酸酯酶通过降解 GhDFR 调节宿主免疫。
The secreted feruloyl esterase of Verticillium dahliae modulates host immunity via degradation of GhDFR.
机构信息
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs and College of Plant Protection, Northwest A&F University, Yangling, China.
Pest & Pathogen Ecology, NIAB East Malling, West Malling, UK.
出版信息
Mol Plant Pathol. 2024 Feb;25(2):e13431. doi: 10.1111/mpp.13431.
Feruloyl esterase (ferulic acid esterase, FAE) is an essential component of many biological processes in both eukaryotes and prokaryotes. This research aimed to investigate the role of FAE and its regulation mechanism in plant immunity. We identified a secreted feruloyl esterase VdFAE from the hemibiotrophic plant pathogen Verticillium dahliae. VdFAE acted as an important virulence factor during V. dahliae infection, and triggered plant defence responses, including cell death in Nicotiana benthamiana. Deletion of VdFAE led to a decrease in the degradation of ethyl ferulate. VdFAE interacted with Gossypium hirsutum protein dihydroflavanol 4-reductase (GhDFR), a positive regulator in plant innate immunity, and promoted the degradation of GhDFR. Furthermore, silencing of GhDFR led to reduced resistance of cotton plants against V. dahliae. The results suggested a fungal virulence strategy in which a fungal pathogen secretes FAE to interact with host DFR and interfere with plant immunity, thereby promoting infection.
阿魏酸酯酶（阿魏酸酯酶，FAE）是真核生物和原核生物中许多生物过程的重要组成部分。本研究旨在探讨 FAE 的作用及其在植物免疫中的调节机制。我们从半活体植物病原菌黄萎病菌中鉴定出一种分泌型阿魏酸酯酶 VdFAE。VdFAE 在 V. dahliae 感染过程中充当重要的毒力因子，引发植物防御反应，包括在本氏烟中引起细胞死亡。VdFAE 的缺失导致乙基阿魏酸酯的降解减少。VdFAE 与棉花蛋白二氢黄酮醇 4-还原酶（GhDFR）相互作用，GhDFR 是植物先天免疫的正向调节因子，促进 GhDFR 的降解。此外，GhDFR 的沉默导致棉花植株对黄萎病菌的抗性降低。结果表明，真菌病原体分泌 FAE 与宿主 DFR 相互作用并干扰植物免疫，从而促进感染，这是一种真菌毒力策略。
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