卵泡液微量元素与体外受精卵巢反应的关系。
Associations between follicular fluid trace elements and ovarian response during in vitro fertilization.
机构信息
Department of Global and Community Health, George Mason University, Fairfax, VA, 22030, United States.
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA, 94158, United States.
出版信息
Environ Res. 2024 Jul 1;252(Pt 1):118801. doi: 10.1016/j.envres.2024.118801. Epub 2024 Mar 29.
INTRODUCTION
Exposure to trace elements has been associated with ovarian response in experimental studies. We conducted a hypothesis-generating study of associations between ovarian follicular fluid (FF) trace elements and measures of ovarian response among women using in vitro fertilization (IVF).
METHODS
We collected ovarian FF specimens from 56 women. We determined concentrations (μg/L) of 11 trace elements using inductively coupled plasma-tandem mass spectrometry. We estimated associations between women's FF trace elements per interquartile range difference, and measures of ovarian response using linear (peak estradiol (E2), baseline anti-mullerian hormone (AMH), and follicle stimulating hormone (FSH)) and negative binomial (baseline antral follicle count (AFC) and oocyte count) regression, adjusting for confounding factors. We used principal component analysis (PCA) to estimate the associations of the FF trace elements mixture. We also explored FF oxidative stress enzymes as causal mediators of the associations.
RESULTS
Higher FF cobalt was associated with greater peak E2 (mean difference = 351.48 pg/mL; 95%CI: 21.76, 724.71) and AFC (rate ratio = 1.14; 95%CI: 1.01, 1.28), and higher FF copper was associated with greater peak E2 (mean difference = 335.66 pg/mL; 95%CI: 81.77, 753.08) and oocyte count (rate ratio = 1.19; 95%CI: 1.02, 1.43). Higher FF mercury was also associated with greater peak E2 (mean difference = 410.70 pg/mL; 95%CI: 61.90, 883.39). Higher FF lead was associated with lesser AFC (rate ratio = 0.85; 95%CI: 0.73, 0.98). Using PCA, the mixture of Sr, Hg, and As was associated with higher peak estradiol, AFC, and oocyte count. FF glutathione peroxidase, paraoxonase, and arylesterase activities were inconsistent mediators of the associations, but the effect estimates were imprecise.
CONCLUSION
Our results suggest that essential and non-essential trace elements in FF were associated with ovarian response during IVF.
简介
在实验研究中，接触微量元素与卵巢反应有关。我们进行了一项假设生成研究，研究了体外受精（IVF）中女性卵巢滤泡液（FF）中的微量元素与卵巢反应测量值之间的关系。
方法
我们从 56 名女性中采集了卵巢 FF 标本。我们使用电感耦合等离子体质谱-串联质谱法测定了 11 种微量元素的浓度（μg/L）。我们使用线性（峰值雌二醇（E2）、基线抗苗勒氏管激素（AMH）和卵泡刺激素（FSH））和负二项式（基线窦卵泡计数（AFC）和卵母细胞计数）回归，调整混杂因素，估计了女性 FF 微量元素的差异与卵巢反应测量值之间的关联。我们使用主成分分析（PCA）来估计 FF 微量元素混合物的相关性。我们还探索了 FF 氧化应激酶作为关联的因果中介。
结果
较高的 FF 钴与较高的峰值 E2（平均差异=351.48pg/mL；95%CI：21.76，724.71）和 AFC（比率比=1.14；95%CI：1.01，1.28）有关，较高的 FF 铜与较高的峰值 E2（平均差异=335.66pg/mL；95%CI：81.77，753.08）和卵母细胞计数（比率比=1.19；95%CI：1.02，1.43）有关。较高的 FF 汞也与较高的峰值 E2 相关（平均差异=410.70pg/mL；95%CI：61.90，883.39）。较高的 FF 铅与 AFC 减少有关（比率比=0.85；95%CI：0.73，0.98）。使用 PCA，Sr、Hg 和 As 的混合物与较高的峰值雌二醇、AFC 和卵母细胞计数有关。FF 谷胱甘肽过氧化物酶、对氧磷酶和芳基酯酶活性是关联的不一致中介，但效应估计值不精确。
结论
我们的结果表明，FF 中的必需和非必需微量元素与 IVF 期间的卵巢反应有关。
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