植物抗毒素樱花素减弱内吞作用并增强对稻瘟病的抗性。

Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast.

作者信息

Jiang Lihui, Zhang Xiaoyan, Zhao Yiting, Zhu Haiyan, Fu Qijing, Lu Xinqi, Huang Wuying, Yang Xinyue, Zhou Xuan, Wu Lixia, Yang Ao, He Xie, Dong Man, Peng Ziai, Yang Jing, Guo Liwei, Wen Jiancheng, Huang Huichuan, Xie Yong, Zhu Shusheng, Li Chengyun, He Xiahong, Zhu Youyong, Friml Jiří, Du Yunlong

机构信息

College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.

Shanxi Agricultural University/Shanxi Academy of Agricultural Sciences. The Industrial Crop Institute, Fenyang, 032200, China.

出版信息

Nat Commun. 2024 Apr 23;15(1):3437. doi: 10.1038/s41467-024-47746-y.

DOI:10.1038/s41467-024-47746-y

PMID:38653755

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11039461/

Abstract

Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription.

摘要

植保素樱花素在抗稻瘟病中发挥作用。然而，樱花素作用的潜在机制仍不清楚。在此，我们报道表达抗性（R）基因的水稻品系中发现含有高水平的樱花素，这与质膜（PM）蛋白的内吞运输减弱相关。外源和内源樱花素均减弱了各种PM蛋白和真菌效应子PWL2的内吞作用。此外，用樱花素处理后，稻瘟病菌摄取到水稻细胞中导致的无毒蛋白AvrCO39的积累减少。对网格蛋白介导的内吞作用（CME）的药理学操作表明该途径是樱花素的作用靶点。实际上，樱花素对CME的减弱足以传递对稻瘟病的抗性。我们的数据揭示了水稻通过提高樱花素水平和抑制病原体效应子的CME来对抗稻瘟病菌的一种机制，这与许多主要通过调节转录起作用的R基因的作用不同。