Multi-layer encapsulation of pumpkin ( L.) seed protein hydrolysate and investigating its release and antioxidant activity in simulated gastrointestinal digestion.

Because of their high protein content, easy access and low cost, pumpkin seeds are a valuable raw material for the preparation of antioxidant protein hydrolysates. Micro-coating is an effective method to protect bioactive compounds against destruction. In order to strengthen the alginate hydrogel network loaded with pumpkin seed protein hydrolysate (PSPH), CMC was added as part of its formulation in the first step, and chitosan coating was used in the second step. Then, swelling amount, release in the simulated gastrointestinal environment (SGI), antioxidant activity after SGI, Fourier transform infrared spectroscopy (FTIR), zeta potential, dynamic light scattering (DLS), polydispersity index (PDI) and scanning electron microscopy (SEM) of the samples were evaluated. The results showed that, the swelling amount of the chitosan-alginate hydrogel was lower than the chitosan-alginate-CMC sample, and with the increase in chitosan concentration, the swelling amount decreased. The release amount in the chitosan-alginate sample was higher than that in the chitosan-alginate-CMC sample, and with the increase in chitosan concentration, the release rate decreased. Also, the amount of release increased with the passage of time. The highest antioxidant activity belonged to the chitosan-alginate sample in SGI, and it increased with increasing the chitosan concentration. All findings demonstrated that the use of multi-component hybrid systems is a useful method for the protection of bioactive compounds against destruction, their antioxidant activities and their release behavior.