lncRNAs LINC00152 和 LARS2-AS1 的诊断价值及其在结核分枝杆菌感染巨噬细胞免疫反应中的调控作用。
Diagnostic value of lncRNAs LINC00152 and LARS2-AS1 and their regulatory roles in macrophage immune response in tuberculosis.
机构信息
Department of Clinical Laboratory, Shanghai Yangsi Hospital, Shanghai, 200126, China.
Department of Ultrasound, Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, China.
出版信息
Tuberculosis (Edinb). 2024 Sep;148:102530. doi: 10.1016/j.tube.2024.102530. Epub 2024 Jun 6.
OBJECTIVES
To determine the usefulness of LINC00152 and LARS2-AS1 as potential biomarkers for latent tuberculosis (LTB) and active tuberculosis (ATB), as well as their effect on Mycobacterium (Mtb) infection.
METHODS
The expression levels of LINC00152 and LARS2-AS1 in the health, patients with LTB and ATB were detected by qRT-PCR. The ROC curves were constructed to show their potential as biomarkers. The intracellular survival assays for Mtb and the levels of immune-related cytokines were determined to discover the effect of LINC00152 and LARS2-AS1 on Mtb infection. The relationships of miR-485-5p with LINC00152 and LARS2-AS1 were explored.
RESULTS
LINC00152 and LARS2-AS1 levels were significantly elevated in patients with ATB and LTB, and Mtb-infected macrophages. LINC00152 and LARS2-AS1 can distinguish the LTB from the health and ATB from LTB. LARS2-AS1 and LINC00152 knock-down reduced the intracellular Mtb survival and induced cellular immune response after Mtb challenge. miR-485-5p was a targeting miRNA for LINC00152 and LARS2-AS1.
CONCLUSIONS
LINC00152 and LARS2-AS1 can be considered as potential biomarkers for tuberculosis disease. LINC00152 and LARS2-AS1 have anti-Mtb effects.
目的
确定 LINC00152 和 LARS2-AS1 作为潜伏性结核病 (LTB) 和活动性结核病 (ATB) 潜在生物标志物的有用性，以及它们对分枝杆菌 (Mtb) 感染的影响。
方法
通过 qRT-PCR 检测健康、LTB 和 ATB 患者中 LINC00152 和 LARS2-AS1 的表达水平。构建 ROC 曲线以显示其作为生物标志物的潜力。进行 Mtb 细胞内存活测定和免疫相关细胞因子水平测定，以发现 LINC00152 和 LARS2-AS1 对 Mtb 感染的影响。探讨 miR-485-5p 与 LINC00152 和 LARS2-AS1 的关系。
结果
ATB 和 LTB 患者以及 Mtb 感染的巨噬细胞中 LINC00152 和 LARS2-AS1 水平显著升高。LINC00152 和 LARS2-AS1 可区分 LTB 与健康人群和 ATB 与 LTB。LARS2-AS1 和 LINC00152 敲低可减少细胞内 Mtb 存活并在 Mtb 攻击后诱导细胞免疫反应。miR-485-5p 是 LINC00152 和 LARS2-AS1 的靶向 miRNA。
结论
LINC00152 和 LARS2-AS1 可被视为结核病的潜在生物标志物。LINC00152 和 LARS2-AS1 具有抗 Mtb 作用。
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