间歇性高氧干预运动训练对雄性小鼠耐力表现和肌肉代谢特性的影响。

Effects of exercise training with intermittent hyperoxic intervention on endurance performance and muscle metabolic properties in male mice.

机构信息

Laboratory of Exercise Physiology, Health and Sports Sciences, Course of Sports Education, Department of Education, Hokkaido University of Education, Iwamizawa, Hokkaido, Japan.

出版信息

Physiol Rep. 2024 Jun;12(12):e16117. doi: 10.14814/phy2.16117.

DOI:10.14814/phy2.16117

PMID:38898524

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11186743/

Abstract

This study aimed to investigate how intermittent hyperoxic exposure (three cycles of 21% O [10 min] and 30% O [15 min]) affects exercise performance in mice. Three hours after the acute exposure, there was an observed increase in mRNA levels of phosphofructokinase (Bayes factor [BF] ≥ 10), mitochondrial transcription factor-A (BF ≥10), PPAR-α (BF ≥3), and PPAR-γ (BF ≥3) in the red gastrocnemius muscle (Gr). Four weeks of exercise training under intermittent (INT), but not continuous (HYP), hyperoxia significantly (BF ≥30) increased maximal exercise capacity compared to normoxic exercise-trained (ET) group. INT group exhibited significantly higher activity levels of 3-hydroxyacyl-CoA-dehydrogenase (HAD) in Gr (BF = 7.9) compared to ET group. Pyruvate dehydrogenase complex activity levels were significantly higher in INT group compared to ET group in white gastrocnemius, diaphragm, and left ventricle (BF ≥3). NT-PGC1α protein levels in Gr (BF = 7.7) and HAD activity levels in Gr (BF = 6.9) and soleus muscles (BF = 3.3) showed a significant positive correlation with maximal work values. These findings suggest that exercise training under intermittent hyperoxia is a beneficial strategy for enhancing endurance performance by improving fatty acid and pyruvic acid utilization.

摘要

这项研究旨在探究间歇性高氧暴露（三个 21% O [10 分钟] 和 30% O [15 分钟] 的循环）如何影响小鼠的运动表现。急性暴露后 3 小时，红比目鱼肌（Gr）中磷酸果糖激酶（贝叶斯因子 [BF] ≥ 10）、线粒体转录因子 A（BF ≥ 10）、过氧化物酶体增殖物激活受体-α（PPAR-α）（BF ≥ 3）和过氧化物酶体增殖物激活受体-γ（PPAR-γ）（BF ≥ 3）的 mRNA 水平增加。与常氧运动训练（ET）组相比，间歇（INT）但非连续（HYP）高氧 4 周运动训练显著（BF ≥ 30）提高了最大运动能力。INT 组 Gr 中 3-羟基酰基辅酶 A 脱氢酶（HAD）的活性水平显著升高（BF = 7.9）与 ET 组相比。INT 组的丙酮酸脱氢酶复合物活性水平在 Gr、白比目鱼肌、横膈膜和左心室均显著高于 ET 组（BF ≥ 3）。Gr 中的 NT-PGC1α 蛋白水平（BF = 7.7）和 Gr 中的 HAD 活性水平（BF = 6.9）和比目鱼肌（BF = 3.3）与最大工作值呈显著正相关。这些发现表明，间歇高氧运动训练是一种通过改善脂肪酸和丙酮酸利用来提高耐力表现的有益策略。