Structural analysis, anti-inflammatory activity of the main water-soluble acidic polysaccharides (AGBP-A3) from L berry.

BACKGROUND

L, widely recognized for its valuable contributions to medicine, has aroused considerable attention globally. Different from the extensive research has been dedicated to the root of , its berry has received relatively scant focus. Given its promising medicinal properties, this study was focused on the structural characterizations and anti-inflammatory potential of acidic polysaccharides from the berry.

MATERIALS AND METHODS

berry was extracted with hot water, precipitated by alcohol, separated by DEAE-52-cellulose column to give a series of fractions. One of these fractions was further purified via Sephadex G-200 column to give three fractions. Then, the main fraction named as AGBP-A3 was characterized by methylation analysis, NMR spectroscopy, etc. Its anti-inflammatory activity was assessed by RAW 264.7 cell model, zebrafish model and molecular docking.

RESULTS

The main chain comprised of -L-Rhap, -D-GalAp and -D-Galp, while the branch consisted mainly of -L-Araf, -D-Glcp, -D-GalAp, -D-Galp. The RAW264.7 cell assay results showed that the inhibition rates against IL-6 and IL-1 secretion at the concentration of 625 ng/mL were 24.83 %, 11.84 %, while the inhibition rate against IL-10 secretion was 70.17 % at the concentration of 312 ng/mL. In the zebrafish assay, the migrating neutrophils were significantly reduced in number, and their migration to inflammatory tissues was inhibited. Molecular docking predictions correlated well with the results of the anti-inflammatory assay.

CONCLUSION

The present study demonstrated the structure of acidic polysaccharides of berry and their effect on inflammation, providing a reference for screening anti-inflammatory drugs.