Green-synthesized silver nanoparticles from as a promising strategy to target quorum sensing and biofilms in .

The global challenge to human health is significantly heightened by the resistance of harmful bacteria to antimicrobial treatments. Given the limited advancement in developing new antimicrobial medications, exploring innovative strategies is imperative to tackle the challenge of resistance to multiple drugs. Furthermore, there is a growing emphasis on the environmentally friendly synthesis of nanoparticles with potent medicinal attributes, specifically those targeting virulence, to combat the rise of multidrug resistance. Focusing on the inhibition of virulence factors and biofilms influenced by quorum sensing has become a promising and novel strategy in the development of anti-infective drugs. An aqueous extract of leaves was used to create green-synthesized silver nanoparticles, or AgNPs. X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and UV-visible absorption spectroscopy were used to characterize the AgNPs. The impact of AgNPs on the virulence factors and biofilms of PAO1, mediated by quorum sensing, was assessed at concentrations below the minimum inhibitory concentration (sub-MIC). Sub-MIC concentrations of Green-synthesized AgNPs inhibited various . virulence factors, including bacterial motility (89 % inhibition), pyocyanin production (81.48 % inhibition), pyoverdin production (55.80 % inhibition), elastase activity (87.43 % inhibition), exoprotease activity (75.60 % inhibition), and rhamnolipid production (71.28 % inhibition). Additionally, these AgNPs demonstrated 80 % inhibition of biofilms. The in vitro efficacy of green-synthesized AgNPs against can be utilized for the creation of alternative therapeutic agents for managing bacterial infections, particularly for topical application in cases such as wound infections. Additionally, they can be used for surface coating to inhibit the attachment of bacteria to medical devices.