从住院患者鲍曼不动杆菌分离株中鉴定 bla、bla、bla 和 bla 碳青霉烯酶基因。
Identification of bla, bla, bla, and bla carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients.
作者信息
Nasrabadi Kosar Salmani, Ahmadi Yasaman, Ghasemi Seyed Mahdi, Shokri Dariush
机构信息
Department of Microbiology, Faculty of Biological Sciences and Technology Shahid Ashrafi, Shahid Ashraf Esfahani University, Isfahan, Iran.
Department of Microbiology, Kish International Branch of Islamic Azad University, Kish, Iran.
出版信息
BMC Res Notes. 2024 Dec 27;17(1):392. doi: 10.1186/s13104-024-07047-5.
OBJECTIVE
The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.
RESULTS
In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla, bla, bla, bla, bla, and bla genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla gene, 23 (92.0%) contained the bla gene, and 1 (4.0%) isolate carried the bla gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla and bla genes (oxacillinase genes) were dominant genes, but bla and bla were not local carbapenem resistant genes in Isfahan.
目的
鲍曼不动杆菌多重耐药（MDR）菌株的增加，尤其是耐碳青霉烯类菌株，给感染治疗带来了挑战。本研究调查了鲍曼不动杆菌的抗生素耐药模式及碳青霉烯耐药基因（氧青霉烯酶和金属β-内酰胺酶）的频率。
结果
在本研究中，从伊朗中部伊斯法罕不同医院的临床样本中收集了100株细菌分离株。在100份血液、尿液、脑脊液（CSF）、伤口和气管样本中，有60株细菌被鉴定为鲍曼不动杆菌。结果显示，所选分离株100%对头孢噻肟、头孢他啶、环丙沙星、哌拉西林-他唑巴坦和美罗培南耐药。根据抗生素耐药模式，选择25株分离株进行针对bla、bla、bla、bla、bla和bla基因的PCR分析。PCR结果显示，在所选分离株中，15株（60.0%）携带bla基因，23株（92.0%）含有bla基因，1株（4.0%）分离株携带bla基因。基于多位点序列分型（MLST）分析，两株耐黏菌素的鲍曼不动杆菌分离株被归类为ST2。ST2克隆代表CC2或国际克隆二中的主要序列类型。结果表明，针对分离株的最佳抗生素是黏菌素。bla和bla基因（氧青霉烯酶基因）是优势基因，但bla和bla不是伊斯法罕本地的碳青霉烯耐药基因。
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