在巴西马拉尼昂州圣路易斯岛双壳贝类软体动物中检测到鼠罗卡病毒：一种新出现的人畜共患病原体的潜在传播途径？

Detection of Rocahepevirus ratti in Bivalve Mollusks from São Luís Island, Maranhão, Brazil: A Potential Transmission Route of an Emerging Zoonotic Pathogen?

作者信息

Figueiredo Andreza Soriano, Negreiros Isabella Rodrigues, do Nascimento E Silva Aldaleia, Salgado Caroline Roberta Soares, Dos Santos Natália Lourenço, Pinto Marcelo Alves, de Carvalho Neta Alcina Vieira, Leite José Paulo Gagliardi, Cantelli Carina Pacheco

机构信息

Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.

Program in Animal Science, State of Maranhão University, São Luís, Maranhão, Brazil.

出版信息

Food Environ Virol. 2025 Jan 4;17(1):11. doi: 10.1007/s12560-024-09624-0.

DOI:10.1007/s12560-024-09624-0

PMID:39754637

Abstract

The attempt to investigate hepatitis E virus (HEV) contamination in naturally growing mangrove bivalve mollusks captured for local sale in a touristic area of Maranhão state in Brazil revealed the detection of rat hepatitis E virus (ratHEV). Using international standard protocols for processing and nucleic acid extraction, we analyzed 89 bivalve samples (Mytella falcata and Crassostrea rhizophorae) with two broadly reactive assays: heminested pan-Hepeviridae (ORF-1) and probe-based HEV-1 to HEV-4 (ORF-2/ORF-3). Heminested reactions presented 2 (2.2%) amplifications of the expected size. Nucleotide identities ranged from 86.6 to 89.0% with ratHEV isolates from wild rats, pigs, one human case and sewage reported in different countries. Regarding the phylogenetic tree, the sequences grouped with genotype HEV-C1. This first report of ratHEV detection in bivalve mollusks may be a starting point for further research on virus variability, distribution, host range and especially the possible role of contaminated shellfish as a vehicle for ratHEV transmission.

摘要

对巴西马拉尼昂州一个旅游区捕获的、用于当地销售的天然生长的红树林双壳贝类软体动物中的戊型肝炎病毒（HEV）污染情况进行调查时，发现了大鼠戊型肝炎病毒（ratHEV）。我们采用国际标准的处理和核酸提取方案，使用两种具有广泛反应性的检测方法，对89个双壳贝类样本（镰状肌蛤和红树蚬）进行了分析：半巢式泛戊型肝炎病毒科（ORF-1）检测法和基于探针的HEV-1至HEV-4（ORF-2/ORF-3）检测法。半巢式反应出现了2次（2.2%）预期大小的扩增。与来自不同国家野生大鼠、猪、1例人类病例和污水中的大鼠戊型肝炎病毒分离株相比，核苷酸同一性范围为86.6%至89.0%。在系统发育树上，这些序列与HEV-C1基因型归为一组。在双壳贝类软体动物中检测到大鼠戊型肝炎病毒的这一首次报告，可能是进一步研究病毒变异性、分布、宿主范围，尤其是受污染贝类作为大鼠戊型肝炎病毒传播载体的可能作用的一个起点。