Phylogenetic and Sequence Analyses of the Variable Region in the Glycoprotein Gene of the Respiratory Syncytial Virus Isolated from Iraqi Patients.

BACKGROUND

Respiratory syncytial virus (RSV) is a common aetiological agent that causes respiratory infections, especially among infants. Identifying circulating RSV genotypes is an essential strategy for understanding the spread of the virus in a certain area. Sequencing the variable regions of the attachment glycoprotein (G) gene of RSV is a quick and direct approach for identifying the genotypes.

METHODS

This study was aimed to sequence the G gene region of RSV isolated from patients admitted to hospitals in Baghdad, Iraq, during the autumn of 2022 and winter of 2023. To achieve this goal, 150 patients with lower respiratory symptoms were screened for RSV infections. RSV-positive samples were detected and confirmed using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) approach, which involved the use of specific TaqMan primer sets targeting RSV subgroups. Then, a G gene region that included hypervariable region 2 (HVR2) was amplified and sequenced using the Sanger sequencing method. Furthermore, molecular and phylogenetic analyses were performed on the G gene region to determine the variability profile of the tested specimens.

RESULTS

There were 41 (26.6%) RSV-positive cases. Of these, the RSV-B subgroup was the most prevalent (82.90%), while the RSV-A subgroup incidence rate was 17.07%. The phylogenetic analysis showed that the RSV-B isolates were related to the BA genotype and shared nucleotide sequence similarities with isolates from India, Australia and the UK. The RSV-A isolates belonged to the ON genotype and had some degree of similarities with isolates from Italy, Tunisia, and France.

CONCLUSION

Seasonal tracking of the RSV isolates would facilitate a better understanding of virus evolution, viral pathogenesis, and genetic diversity.