targeted by miR-126-x and miR-21-y responds to infection in .

INTRODUCTION

The Hong Kong oyster (), as the main marine aquaculture shellfish in the South China Sea, not only has high economic and ecological value, but also is an ideal model for conducting research on pathogen-host interactions. In the cultivation process of , there is a challenge posed by . To improve the antibacterial strains of , we have studied the gene associated with immunity, .

METHODS AND FINDINGS

In this study, we cloned the sequence of the , using the RACE technique. It has a total of 2081 bp and contains a 5'-UTR of 55 bp and a 3'-UTR of 547 bp. The ChPDIA3 gene sequence has an ORF frame that is 1479 bp in length and encodes 492 amino acids. Analysis of the phylogenetic tree constructed by Neighbor Joining method showed that clustered with other shellfishes into a single unit, which was consistent with the law of species evolution.

DISCUSSION

The highest expression of was detected in gill tissues of the using RT-qPCR, and significantly higher expression in and LPS infection than Poly(I:C) (<0.05). This may indicate that is primarily involved in the immune response against bacterial infections in the . The binding sites of miR-126-x, miR-21-y and were detected using dual luciferase experiments, respectively. The results showed that both miR-126-x and miR-21-y inhibited the 3'-UTR region of . This suggested that both miR-126-x and miR-21-y inhibited expression. This study will help to further understand the function of in response to pathogen infection, thus providing new ideas for understanding the resistance and adaptation of the to infection.