通过SALSA方法进行的全糖组分析进展，包括唾液酸化亚聚糖异构体分析。
Advances in total glycomic analysis including sialylated sub-glycan isomers by SALSA method.
作者信息
Kurogochi Masaki, Suzuki Chiharu, Hanamatsu Hisatoshi, Furukawa Jun-Ichi
机构信息
Institute for Glyco-core Research (iGCORE) Nagoya University Nagoya 464-8601 Japan.
Department of Orthopaedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
出版信息
BBA Adv. 2025 Jan 28;7:100144. doi: 10.1016/j.bbadva.2025.100144. eCollection 2025.
All eukaryotic cell surfaces are coated with various types of glycans, which are essential molecules in biological events. In this review, we summarize recent integrated glycomics studies using various biological samples. We introduce an improved sialic acid linkage-specific alkylamidation (SALSA) method for sialylated glycan analysis and an automated glycosphingolipid-glycan preparation system for large-scale glycomic analysis of human plasma/serum. Finally, we explain the importance of integrated glycomics of glycoconjugates through total glycomic analysis of human serum and mouse brain tissue, and discuss prospects for exploring glycans as effective biomarkers of biological phenomena.
所有真核细胞表面都覆盖着各种类型的聚糖，这些聚糖是生物事件中的重要分子。在本综述中，我们总结了近期使用各种生物样本进行的综合糖组学研究。我们介绍了一种用于唾液酸化聚糖分析的改进的唾液酸连接特异性烷基酰胺化（SALSA）方法，以及一种用于人血浆/血清大规模糖组学分析的自动化糖鞘脂-聚糖制备系统。最后，我们通过对人血清和小鼠脑组织的全糖组分析来解释糖缀合物综合糖组学的重要性，并讨论探索聚糖作为生物现象有效生物标志物的前景。
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