Fluorescence spectroscopy as a novel tool in hematological diagnostics.

The present paper explores the application of fluorescence spectroscopy in erythrocyte analysis, aiming to enhance spectral diagnostics in biomedical research. The primary objective is to develop innovative methodologies for improving the precision of hematological diagnostics and disease monitoring. Utilizing 3D fluorescence spectroscopy and excitation/emission wavelength mapping, erythrocyte samples are examined across multiple wavelengths, generating distinct spectral profiles that reveal biochemical composition, oxygenation status, and metabolic alterations. Advanced data analysis enables the identification of pathological changes in erythrocytes, contributing to a more comprehensive diagnostic approach. Additionally, this study integrates fluorescence spectroscopy with traditional clinical hematological analysis, comparing spectroscopic findings with complete blood count parameters for two patients. Blood samples were subjected to fluorescence analysis under deuterium, halogen, and ultraviolet excitation sources, allowing for a detailed correlation between spectroscopic biomarkers (hemoglobin, deoxyhemoglobin, and plasma characteristics) and clinical parameters (hemoglobin concentration, hematocrit, and red blood cell indices). The findings demonstrate that fluorescence spectroscopy provides complementary diagnostic insights, detecting subtle physiological variations in blood composition that conventional methods might overlook. By integrating these two diagnostic approaches, this research highlights the potential of fluorescence-based techniques as a noninvasive and efficient tool for hematological diagnostics.