用于黄斑变性研究的诱导多能干细胞衍生的视网膜色素上皮细胞

Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells for the Study of Macular Degeneration.

作者信息

Neiteler Almar, Borooah Shyamanga, Ross James A

机构信息

Tissue Injury and Repair Group, Chancellor's Building, University of Edinburgh, Edinburgh, UK.

Shiley Eye Institute, University of California San Diego, San Diego, CA, USA.

出版信息

Methods Mol Biol. 2025;2924:101-112. doi: 10.1007/978-1-0716-4530-7_8.

DOI:10.1007/978-1-0716-4530-7_8

PMID:40307638

Abstract

We have developed a method to reliably generate retinal pigment epithelium (RPE) in bulk from human induced pluripotent stem cells (iPSCs). These iPSC-RPE showed a typical RPE-like morphology with pigmentation and expressed typical RPE markers. The iPSC-RPE also show a transepithelial electrical resistance and can phagocytose photoreceptor outer segments, indicating that they are also functional. Large quantities of iPSC-RPE can be generated with this method through serial expansion for disease modeling and high-throughput screenings.

摘要

我们已经开发出一种方法，可从人类诱导多能干细胞（iPSC）大量可靠地生成视网膜色素上皮（RPE）。这些iPSC-RPE呈现出带有色素沉着的典型RPE样形态，并表达典型的RPE标志物。iPSC-RPE还表现出跨上皮电阻，并且能够吞噬光感受器外段，这表明它们也具有功能。通过连续传代扩增，利用这种方法可以生成大量的iPSC-RPE，用于疾病建模和高通量筛选。