利用ITS和rbcL测序确定油橄榄（L.）基因型间遗传多样性及亲缘关系的能力。
Ability to use ITS and rbcL sequencing for determination of the genetic diversity and relationships among olive ( L.) genotypes.
作者信息
Firouzi Sobhan, Solouki Mahmood, Fazeli-Nasab Bahman, Salehi-Sardoei Ali, Hatami Mehrnaz, Ghorbanpour Mansour, Zhang Baohong
机构信息
Department of Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran.
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
出版信息
3 Biotech. 2025 Jun;15(6):161. doi: 10.1007/s13205-025-04336-z. Epub 2025 May 12.
UNLABELLED
Olives are of considerable economic and commercial importance and are mostly used in both daily life and industries. The DNA barcode method has a lot of potential for reviving the science of arithmetics and traditional biodiversity studies, so it has been widely used on plants and for classification and arithmetic purposes. In this study, we sequenced seven different olive genotypes ( cv. Olive yellow, cv. Oliy, cv. Roodbar Oily, cv. Mari, cv. Fishemi, cv. Manzanila, cv. Koroneiki) to study their diversity and evolution. The data were analyzed using Clustalw2 and BioEdit software. The homology rate of rbcL and ITS sequences was all in the range of 97-100%. It was identified, for ITS, 1,059 genetic luci (580 luci without deletion and addition and 479 luci with deletion and addition (328 luci polymorphs, 151 monomorphs), 217 singletons, and for rbcL, 565 genetic luci with ( 60 luci without deletion and addition and 505 luci with deletion and addition (2 luci with polymorphs, 503 monomorphs), 1 singleton. It was determined that the number of four haplotypes (haplotype diversity index = 0.80) was determined for ITS and three haplotypes (haplotype diversity index = 0.71) for RBCL. The results indicated that in the nucleotide sequence of the ITS gene among olive varieties, guanine was the most abundant base at 28.7%, while adenine had the lowest abundance at 5%. In contrast, the rbcL gene showed that thymine was the most abundant base at 29.8%, with cytosine being the least abundant at 20.6%. Estimates of nucleotide transitions in the ITS gene revealed a high frequency of pyrimidine transitions, with a thymine-to-cytosine transition rate of 16.84% and a cytosine-to-thymine transition rate of 11.63%. The ITS primer successfully identified and separated only six genotypes, whereas rbcL identified all seven genotypes. Although the success rates of 60-70% for both ITS and rbcL may not seem particularly high, they still significantly contribute to large-scale biodiversity inventories, especially for olive species.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-025-04336-z.
未标注
橄榄具有相当重要的经济和商业价值，在日常生活和工业中都有广泛应用。DNA条形码方法在复兴分类学和传统生物多样性研究方面具有很大潜力，因此已广泛应用于植物分类和鉴定。在本研究中，我们对7种不同的橄榄基因型（橄榄黄、奥利、鲁德巴尔油橄榄、玛丽、菲舍米、曼萨尼拉、科罗内基）进行测序，以研究它们的多样性和进化。使用Clustalw2和BioEdit软件对数据进行分析。rbcL和ITS序列的同源率均在97%-100%之间。ITS序列共鉴定出1059个基因位点（580个无缺失和添加的位点，479个有缺失和添加的位点（328个多态性位点，151个单态性位点），217个单例；rbcL序列共鉴定出565个基因位点（60个无缺失和添加的位点，505个有缺失和添加的位点（2个多态性位点，503个单态性位点），1个单例。确定ITS有4种单倍型（单倍型多样性指数=0.80），rbcL有3种单倍型（单倍型多样性指数=0.71）。结果表明，在橄榄品种的ITS基因核苷酸序列中，鸟嘌呤含量最高，为28.7%，而腺嘌呤含量最低，为5%。相比之下，rbcL基因显示胸腺嘧啶含量最高，为29.8%，胞嘧啶含量最低，为20.6%。ITS基因核苷酸转换的估计显示嘧啶转换频率较高，胸腺嘧啶到胞嘧啶的转换率为16.84%，胞嘧啶到胸腺嘧啶的转换率为11.63%。ITS引物仅成功鉴定并区分了6种基因型，而rbcL鉴定出了所有7种基因型。虽然ITS和rbcL的成功率60%-70%看起来不是特别高，但它们仍对大规模生物多样性清查有显著贡献，尤其是对橄榄物种。
补充信息
在线版本包含可在10.1007/s13205-025-04336-z获取的补充材料。
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