从诱导多能干细胞分化间充质基质/干细胞

Differentiation of Mesenchymal Stromal/Stem Cells from Induced Pluripotent Stem Cells.

作者信息

Marais Alex Leah, Logan Murray Glen, Mellet Juanita, Ambele Melvin A, Pepper Michael S

机构信息

Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

出版信息

Methods Mol Biol. 2025;2938:155-170. doi: 10.1007/978-1-0716-4607-6_18.

DOI:10.1007/978-1-0716-4607-6_18

PMID:40445291

Abstract

Mesenchymal stromal/stem cells (MSCs) possess unique immunomodulatory and regenerative properties, making them ideal candidates for addressing a variety of disorders. With an increasing number of clinical trials, reliable sources are needed to obtain sufficient cell numbers and to circumvent inherent limitations associated with primary cells. Induced pluripotent stem cells (iPSCs) can be differentiated into various somatic cell types and are able to proliferate indefinitely in culture. Differentiation of iPSCs presents a viable alternative in order to create target cell types for both proof-of-concept research and therapeutic applications. In this chapter, we present a protocol to derive MSC-like cells from iPSCs as well as methods of characterizing these cells.

摘要

间充质基质/干细胞（MSCs）具有独特的免疫调节和再生特性，使其成为治疗多种疾病的理想候选者。随着临床试验数量的增加，需要可靠的来源来获得足够数量的细胞，并规避与原代细胞相关的固有局限性。诱导多能干细胞（iPSCs）可以分化为各种体细胞类型，并能够在培养中无限增殖。iPSCs的分化为概念验证研究和治疗应用创造目标细胞类型提供了一种可行的替代方法。在本章中，我们介绍了一种从iPSCs中衍生出类MSC细胞的方案以及这些细胞的表征方法。