下一代谱系追踪及其在揭示发育过程中的应用。
Next generation lineage tracing and its applications to unravel development.
作者信息
Short Spencer, García-Tejera Rodrigo, Schumacher Linus J, Coutu Daniel L
机构信息
Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Department of Cellular & Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
出版信息
NPJ Syst Biol Appl. 2025 Jun 5;11(1):60. doi: 10.1038/s41540-025-00542-w.
Lineage tracing remains an essential approach towards understanding cell fate, tissue formation, and human development. Herein, we review advancements in lineage tracing techniques, the integration of sequencing and imaging technologies, and computational tools for analysis. We report on recent lineage tracing applications, including integrative techniques (MADM-CloneSeq), in situ hybridization (DART-FISH), and modern workflows (BaSISS), which hold an essential role in our aim to unravel lineage hierarchies and, ultimately, human development.
谱系追踪仍然是理解细胞命运、组织形成和人类发育的重要方法。在此，我们回顾了谱系追踪技术、测序与成像技术的整合以及分析用计算工具的进展。我们报告了近期谱系追踪的应用，包括整合技术（MADM-CloneSeq）、原位杂交（DART-FISH）和现代工作流程（BaSISS），这些在我们揭示谱系层次结构乃至人类发育的目标中发挥着重要作用。
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