光鳃金龟（Kibakoganea tamdaoensis）（鞘翅目：金龟科：丽金龟亚科）的染色体水平基因组

Chromosome-level genome of the shining chafers Kibakoganea tamdaoensis (Coleoptera: Scarabaeidae: Rutelinae).

作者信息

Shen Rongrong, Sylvester Terrence, Ding Qiang, Yang Xinwen, Tong Yijie, Liu Ning, Wang Can, Xiao Yansong, Huang Chunyang, Wu Shaolong, Bai Ming

机构信息

State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Department of Biological Sciences, University of Memphis, Memphis, TN, 38152, USA.

出版信息

Sci Data. 2025 Aug 1;12(1):1345. doi: 10.1038/s41597-025-05657-7.

DOI:10.1038/s41597-025-05657-7

PMID:40751049

Abstract

The beetle subfamily Rutelinae (shining leaf chafers) in the family Scarabaeidae is famous for its colorful body and phytophagous habitat. Among these shining leaf chafers, Kibakoganea tamdaoensis Miyake et Muramoto, 1992 is one with ornamental value because of their enlarged and horn-like mandibles. Here we generated high-quality genome assembly of KTAMD, integrating PacBio HiFi and Hi-C, and annotated with RNA sequence data. The resulting assembly is 605.37 Mb, of which 96.09% is anchored into 10 chromosomes. The scaffold N50 is 54.00 Mb, and BUSCO completeness is 96.6%. Repetitive elements span 49.43% of the genome. Our annotation pipeline identified 984 noncoding RNAs and predicted 12,945 protein-coding genes in the genome (96.40% BUSCO completeness). As the chromosome-scale genome from the subfamily Rutelinae, the KTAMD genome provides important new insights into scarabae genome evolution and provides a new vantage point from which to study the evolution of specialized beetles.

摘要

金龟科丽金龟亚科（亮叶金龟）以其色彩斑斓的身体和植食性栖息地而闻名。在这些亮叶金龟中，1992年发现的谭道奇巴丽金龟（Kibakoganea tamdaoensis Miyake et Muramoto）因其膨大的角状上颚而具有观赏价值。在此，我们整合了PacBio HiFi和Hi-C技术，并结合RNA序列数据，生成了高质量的谭道奇巴丽金龟基因组组装。最终的组装基因组大小为605.37 Mb，其中96.09%被锚定到10条染色体上。支架N50为54.00 Mb，BUSCO完整性为96.6%。重复元件占基因组的49.43%。我们的注释流程在基因组中鉴定出984个非编码RNA，并预测了12,945个蛋白质编码基因（BUSCO完整性为96.40%）。作为丽金龟亚科的染色体水平基因组，谭道奇巴丽金龟基因组为金龟子基因组进化提供了重要的新见解，并为研究特殊甲虫的进化提供了新的视角。