泛酸盐调节疟疾媒介斯氏按蚊的摄食和繁殖，其模式取决于补充方案和亲本营养。

Pantothenate regulates feeding and reproduction in the malaria vector Anopheles stephensi, with patterns dependent on supplementation scheme and parental nutrition.

作者信息

Dobson Megan E, Kaylor Hannah L, Pruett Sydney L, Brady Jessica, Savoie-Penton Kayla, Isoe Jun, Debebe Yared, Riehle Michael A, Luckhart Shirley

机构信息

Department of Biological Sciences, University of Idaho, Moscow, ID, 83843, USA.

Department of Entomology, Plant Pathology, and Nematology, University of Idaho, Moscow, ID, 83843, USA.

出版信息

Parasit Vectors. 2025 Aug 4;18(1):334. doi: 10.1186/s13071-025-06959-w.

DOI:10.1186/s13071-025-06959-w

PMID:40760026

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12323043/

Abstract

BACKGROUND

Pantothenate (Pan), or vitamin B5, is the substrate for biosynthesis of coenzyme A (CoA), an essential cellular cofactor involved in many metabolic processes. Our previous studies demonstrated that Pan availability influences a broad range of traits across multiple species, including malaria parasite development in the mosquito Anopheles stephensi. Accordingly, restricting Pan availability during parasite development may be a viable strategy for malaria control. However, the physiological roles of Pan in A. stephensi remain unclear. In these studies, we investigated the effects of Pan supplementation on this globally important malaria vector.

METHODS

Female A. stephensi were supplemented with Pan via either water, which, similar to plant nectar, is directed to the crop and then slowly released into the midgut, or blood, which transits directly to the midgut for digestion. The effects of provisioning on subsequent blood feeding behavior, reproduction, and offspring sex ratio were assessed. We evaluated these traits across multiple generations, with and without additional supplementation of offspring.

RESULTS

Our findings revealed that Pan regulates vectorially important traits in concentration-, delivery-, and age-dependent ways. The greatest effects of Pan provisioning were on reproduction. The unsupplemented offspring of mothers supplemented with Pan via water exhibited increased fecundity, indicating transgenerational effects from supplemented mothers. However, when Pan was provisioned in blood, only mothers and their supplemented offspring exhibited altered reproduction.

CONCLUSIONS

Our work establishes the importance of Pan in A. stephensi reproduction and provides a foundation for investigating the transgenerational effects of Pan and CoA on mosquito physiology. These observations suggest that targeting Pan-CoA biology in Anopheles spp. could provide opportunities for novel mosquito control strategies.

摘要

背景

泛酸（Pan），即维生素B5，是辅酶A（CoA）生物合成的底物，CoA是一种参与许多代谢过程的必需细胞辅因子。我们之前的研究表明，泛酸的可利用性会影响多个物种的广泛性状，包括疟原虫在斯氏按蚊体内的发育。因此，在寄生虫发育过程中限制泛酸的可利用性可能是控制疟疾的一种可行策略。然而，泛酸在斯氏按蚊中的生理作用仍不清楚。在这些研究中，我们调查了补充泛酸对这种全球重要疟疾传播媒介的影响。