Optimizing the Bioprocesses of Bacteriocin Production in HD1.7 by the "Acetate Switch": Novel Insights into the Labor Division Between Energy Metabolism, Quorum Sensing, and Acetate.

Acetate may act as a signaling molecule, regulating Paracin 1.7 production via quorum sensing (QS) in HD1.7. The "acetate switch" phenomenon requires mechanistic exploration to optimize Paracin 1.7 production. The "acetate switch" phenomenon delays with higher glucose levels (30 h, 36 h, and 96 h). Before the occurrence of the "acetate switch", the ATP content increases and peaks at the "acetate switch" point and the NAD/NADH ratio decreases, indicating energy changes. Moreover, the QS genes used for the pre-regulation of bacteriocin, such as , , were highly expressed. After the "acetate switch", the ATP content decreased and the QS genes for the post-regulation of bacteriocin were highly expressed, such as and /. The "acetate switch" could act as an energy switch, regulating bacterial growth and QS genes. Before and after the "acetate switch", some metabolic pathways were significantly altered according to the transcriptomic analysis by HD1.7 and HD1.7-Δ. In this study, acetate was used as an input signal to regulate the two-component system, significantly influencing the bacteriocin expression system. And this study clarifies the roles of acetate, energy, and quorum sensing in promoting Paracin 1.7 production, providing a theoretical basis for optimizing the bacteriocin fermentation process of HD1.7.