牙胶尖根管充填剂污染风险评估。

Evaluation of the risk of cross-contamination of gutta-percha cartridges.

作者信息

Smith Whitney A, Hutchison Carissa A, Scott Rodney V, Hinman Susan E, Osborne Nancy H, Hamlin Nicholas J, Frank Kristi L

机构信息

Dental Corps, United States Navy, and Endodontics Department, Naval Postgraduate Dental School, Naval Medical Leader and Professional Development Command, and Postgraduate Dental College, Uniformed Services University of the Health Sciences, Bethesda, MD.

Department of Microbiology and Immunology, School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD.

出版信息

JADA Found Sci. 2023;2. doi: 10.1016/j.jfscie.2023.100021. Epub 2023 Mar 21.

DOI:10.1016/j.jfscie.2023.100021

PMID:40808878

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12345616/

Abstract

BACKGROUND

Gutta-percha (GP) cartridges are intended for a single patient and are not considered sterile. Providers may use these cartridges in multiple canals of a single tooth or multiple teeth on the same patient without disinfection between uses, creating the potential for bacteria to be transferred between canal systems. is a hardy bacterial species often isolated from failed root canal treatments. This study tested if GP cartridges have the potential to cross-contaminate root canals with . Methods for GP cartridge disinfection were also evaluated.

METHODS

Twenty-three-gauge medium-body GP cartridges placed in an obturation unit were purposefully contaminated with 1 of 3 concentrations of strain OG1RF. Contaminated cartridges were either (1) wiped with an alcohol swab, (2) heated with GP extrusion while in the bacterial culture, (3) heated with GP extrusion while in the bacterial culture and then wiped with an alcohol swab, or (4) not disinfected after removal from the bacterial culture (control). Bacteria were dislodged into a fresh medium by sonication, and the number of viable bacteria recovered from the tips was measured.

RESULTS

Viable cultures of could not be recovered consistently from tips in the control and disinfection treatment conditions.

CONCLUSIONS

Cross-contamination of root canals with with the GP cartridge is unlikely.

摘要

背景

牙胶尖（GP）根管充填剂专为单一患者设计，并非无菌产品。临床医生可能会在同一患者的单颗牙齿的多个根管或多颗牙齿上使用这些根管充填剂，且在使用之间不进行消毒，这就增加了细菌在根管系统之间传播的可能性。粪肠球菌是一种常在失败的根管治疗中分离出的顽强细菌。本研究测试了牙胶尖根管充填剂是否有可能导致粪肠球菌在根管间交叉污染。同时也评估了牙胶尖根管充填剂的消毒方法。

方法

将23号中型牙胶尖根管充填剂置于根管充填装置中，分别用3种浓度的粪肠球菌菌株OG1RF中的一种进行有意污染。被污染的根管充填剂分别进行以下处理：（1）用酒精棉球擦拭；（2）在细菌培养过程中通过挤出牙胶尖进行加热；（3）在细菌培养过程中通过挤出牙胶尖进行加热，然后用酒精棉球擦拭；或（4）从细菌培养物中取出后不进行消毒（对照组）。通过超声处理将细菌释放到新鲜培养基中，并测量从根管充填剂尖端回收的活菌数量。