两性霉素甲酯的佐剂作用与促细胞分裂作用之间的关系。

The relationship between adjuvant and mitogenic effects of amphotericin methyl ester.

作者信息

Little J R, Abegg A, Plut E

出版信息

Cell Immunol. 1983 Jun;78(2):224-35. doi: 10.1016/0008-8749(83)90277-0.

PMID:6190575

Abstract

The antifungal polyene amphotericin B (AmB) and its methyl ester derivative (AME) both show potent murine immunostimulant as well as B-cell activating effects. Under certain experimental conditions, AME is a much more potent polyclonal B-cell activator (PBA) than AmB. Notable features of the murine B-cell stimulation induced by AME include: (i) High concentrations of AME (50-100 microgram/ml) are required and even at this level exhibit little or no spleen cell toxicity. (ii) Several lines of evidence suggest that the B-cell activating properties of AME are not involved in the cellular mechanism of adjuvant activity in vivo. (iii) There is a strong correlation between the magnitude of the in vitro PBA effects and the in vivo adjuvant effects of AME in a survey of different mouse strains. This evidence suggests that there is genetic control of the murine lymphoid cell-stimulatory effects of AME and that a small number of genes determines the responsive phenotype.

摘要

抗真菌多烯两性霉素B（AmB）及其甲酯衍生物（AME）均表现出强大的小鼠免疫刺激作用以及B细胞激活作用。在某些实验条件下，AME作为多克隆B细胞激活剂（PBA）比AmB更为有效。AME诱导的小鼠B细胞刺激的显著特征包括：（i）需要高浓度的AME（50 - 100微克/毫升），即使在此水平，对脾细胞的毒性也很小或没有毒性。（ii）多项证据表明，AME的B细胞激活特性不参与其体内佐剂活性的细胞机制。（iii）在对不同小鼠品系的调查中，AME的体外PBA效应大小与体内佐剂效应之间存在很强的相关性。这一证据表明，AME对小鼠淋巴细胞的刺激作用存在基因控制，并且少数基因决定了反应表型。