[蜡样芽孢杆菌细胞外磷脂酶C（卵磷脂酶）的生物合成取决于营养培养基组成和pH值]

[Biosynthesis of extracellular phospholipase C (lecithinase) from Bacillus cereus depending on the nutrient medium composition and pH].

作者信息

Gerasimene G B, Makariunaite Iu P, Kulene V V, Glemzha A A

出版信息

Prikl Biokhim Mikrobiol. 1980 Jul-Aug;16(4):523-7.

Abstract

The nutrient medium to provide rapid growth of microbial cells of Bacillus cereus str. 504, and biosynthesis of extracellular phospholipase C(EC 3.1.4.3) was selected. The nutrient medium contained acidic casein hydrolyzate, yeast extract or enzymatic hydrolyzate of fodder yeast, glucose, NaCl, NaHCO3, and Na2HPO4. The activity of secreted phospholipase C reached maximum at pH 6.3--6.6.

摘要

筛选出了一种营养培养基，用于蜡状芽孢杆菌菌株504的微生物细胞的快速生长以及细胞外磷脂酶C（EC 3.1.4.3）的生物合成。该营养培养基含有酸性酪蛋白水解物、酵母提取物或饲料酵母酶解物、葡萄糖、氯化钠、碳酸氢钠和磷酸氢二钠。分泌的磷脂酶C的活性在pH 6.3 - 6.6时达到最大值。