一种用于分离多形核粒细胞的快速密度梯度技术。

A rapid density gradient technique for separating polymorphonuclear granulocytes.

作者信息

Cabanis A, Gressier B, Lebegue S, Brunet C, Dine T, Luyckx M, Cazin M, Cazin J C

机构信息

Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Lille, France.

出版信息

APMIS. 1994 Feb;102(2):119-21. doi: 10.1111/j.1699-0463.1994.tb04855.x.

DOI:10.1111/j.1699-0463.1994.tb04855.x

PMID:8167007

Abstract

A gradient separation technique followed by isotonic ammonium chloride haemolysis was compared with two methods for the isolation of polymorphonuclear neutrophils from blood. This technique provided a high yield, excellent purity without lymphocyte and erythrocyte contamination, and made it possible to isolate more than 50 x 10(6) human neutrophils from 15 ml of blood. The polymorphonuclear neutrophils isolated in this way were capable of generating a large amount of reactive oxygen species. This technique for the separation of polymorphonuclear neutrophils is an effective method for in vitro studies.

摘要

将一种梯度分离技术与等渗氯化铵溶血法相结合，与两种从血液中分离多形核中性粒细胞的方法进行了比较。该技术产量高、纯度优异，无淋巴细胞和红细胞污染，并且能够从15毫升血液中分离出超过50×10⁶个人类中性粒细胞。以这种方式分离出的多形核中性粒细胞能够产生大量活性氧物质。这种分离多形核中性粒细胞的技术是一种用于体外研究的有效方法。