用碘-123-BMIPP评估犬模型中的心肌存活情况。

Evaluation of myocardial viability with iodine-123-BMIPP in a canine model.

作者信息

Nohara R, Okuda K, Ogino M, Hosokawa R, Tamaki N, Konishi J, Fujibayashi Y, Yonekura Y, Fujita M, Sasayama S

机构信息

Department of Internal Medicine, Faculty of Pharmaceutical Sciences, Kyoto University Hospital, Japan.

出版信息

J Nucl Med. 1996 Aug;37(8):1403-7.

PMID:8708784

Abstract

UNLABELLED

The tracer 123I-BMIPP was examined for its ability to reflect myocardial lipid metabolism. Studies in mice indicate that myocardial BMIPP uptake correlates with ATP content. Details, however, of myocardial accumulation in the ischemic period with either infarct or ischemia are not well documented.

METHODS

Sixteen adult mongrel dogs were investigated. The occluded left anterior descending artery (LAD) alone was reperfused to make the ischemic area, and the first diagonal branch of the LAD was kept occluded to make the infarct area. Regional wall motion was evaluated by echocardiography in the short-axial view from the epicardium. Tissue blood flow was calculated using nonradioactive colored microspheres. Changes in blood glucose levels, lipid levels and lactate extraction were examined in blood collected from the aorta and great cardiac vein (GCV). The ATP concentration and BMIPP count were determined by high-performance liquid chromatography and gamma-counter, respectively.

RESULTS

Two hours after reperfusion, blood flow decreased to 20% +/- 5% in the infarct area and 64% +/- 9% in the ischemic area (p < 0.05), despite comparable wall-motion reduction (32% +/- 5% and 42% +/- 12% in the infarct and ischemic areas, respectively). BMIPP content and ATP concentration showed parallel reduction: 40% +/- 7% and 75% +/- 4% (p < 0.05) of BMIPP and 32% +/- 9% and 69% +/- 7% (p < 0.05) of ATP in the infarct and ischemic areas, respectively. The nonesterified fatty acid extraction, defined as flow x ([artery] - [GCV]), decreased to 87% +/- 5.6% during occlusion and 75% +/- 20.1% 2 hr after reperfusion, as compared with the control value.

CONCLUSION

BMIPP uptake correlated well with lipid metabolism and tissue ATP levels and may prove useful in differentiating myocardial infarction from ischemia in the acute phase of ischemic episodes.

摘要

未标记

对示踪剂123I - BMIPP反映心肌脂质代谢的能力进行了研究。对小鼠的研究表明，心肌对BMIPP的摄取与ATP含量相关。然而，关于梗死或缺血期心肌蓄积的详细情况尚无充分记录。

方法

对16只成年杂种犬进行研究。仅对闭塞的左前降支动脉（LAD）进行再灌注以形成缺血区，而LAD的第一对角支保持闭塞以形成梗死区。通过超声心动图在短轴视图中从心外膜评估局部壁运动。使用非放射性彩色微球计算组织血流量。检测从主动脉和大冠状静脉（GCV）采集的血液中血糖水平、脂质水平和乳酸摄取的变化。分别通过高效液相色谱法和γ计数器测定ATP浓度和BMIPP计数。

结果

再灌注2小时后，梗死区血流量降至20%±5%，缺血区降至64%±9%（p<0.05），尽管壁运动减少程度相当（梗死区和缺血区分别为32%±5%和42%±12%）。BMIPP含量和ATP浓度呈平行降低：梗死区和缺血区的BMIPP分别为40%±7%和75%±4%（p<0.05），ATP分别为32%±9%和69%±7%（p<0.05）。非酯化脂肪酸摄取，定义为血流量×（[动脉] - [GCV]），与对照值相比，在闭塞期间降至87%±5.6%，再灌注2小时后降至75%±20.1%。