人肝细胞癌中c-myc基因无基因扩增情况下的过表达

Overexpression of c-myc gene without gene amplification in human hepatocellular carcinoma.

作者信息

Wu B, Wang M, You W, Yang S

机构信息

General Hospital of PLA, Beijing.

出版信息

Chin Med J (Engl). 1996 Dec;109(12):922-5.

PMID:9275322

Abstract

OBJECTIVE

To clarify the relationship between c-myc gene amplification and expression in human hepatocellular carcinoma (HCC) tissue.

METHODS

Levels of amplification, transcription and P62 myc expression of c-myc gene in tumor tissues and corresponding non-tumor tissues were systematically studied using Dot blot hybridization, in situ hybridization and immunohistochemical technique, respectively. The 14 pairs of specimens were resected from patients with HCC.

RESULTS

The amplification of c-myc gene was not found in tumor and non-tumor tissues. Overexpression of c-myc mRNA transcription was detected in 11 tumor tissues and 10 non-tumor tissues. The positive rate of P62 myc was 85.7% (12/14) in tumor tissues and 92.9% (13/14) in non-tumor tissues, respectively. A close correlation was noticed between c-myc mRNA and P62 myc expression. The results suggested that c-myc gene overexpression was not related to its amplification.

CONCLUSIONS

The overexpression of c-myc mRNA and gene product without amplification may be characteristic in HCC.

摘要

目的

阐明人肝细胞癌（HCC）组织中c-myc基因扩增与表达之间的关系。

方法

分别采用斑点杂交、原位杂交和免疫组化技术，系统研究肿瘤组织及相应非肿瘤组织中c-myc基因的扩增、转录水平及P62 myc表达。从肝癌患者中切除14对标本。

结果

在肿瘤组织和非肿瘤组织中均未发现c-myc基因扩增。在11例肿瘤组织和10例非肿瘤组织中检测到c-myc mRNA转录的过表达。肿瘤组织中P62 myc的阳性率分别为85.7%（12/14），非肿瘤组织中为92.9%（13/14）。c-myc mRNA与P62 myc表达之间存在密切相关性。结果表明，c-myc基因过表达与其扩增无关。