在反密码子环内替换的tRNA变体的密码子阅读特性。

Codon reading properties of tRNA variants substituted within the anticodon loop.

作者信息

Ouchi R, Takai K, Yokoyama S, Takaku H

机构信息

Department of Industrial Chemistry, Faculty of Technology, Chiba Institute of Technology, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):115-6.

PMID:9586026

Abstract

Effects of base substitution within tRNA anticodon loop on the codon reading activities were quantitatively analyzed with the use of a set of unmodified tRNA molecules with GGA anticodon. The first (position 32) and the last (position 38) nucleotides of the anticodon loop of the wild-type molecule was changed from C32A38 to U32A38, U32G38, and C32G38. The codon reading activities of these variants relative to that of the wild type molecule were measured in a cell-free translation system. The reading of both the UCU and UCC codons were lower in all the three variants than in the wild-type molecule.

摘要

利用一组具有GGA反密码子的未修饰tRNA分子，定量分析了tRNA反密码子环内碱基替换对密码子阅读活性的影响。野生型分子反密码子环的第一个（第32位）和最后一个（第38位）核苷酸从C32A38变为U32A38、U32G38和C32G38。在无细胞翻译系统中测量了这些变体相对于野生型分子的密码子阅读活性。所有这三个变体对UCU和UCC密码子的阅读都低于野生型分子。